Cryptosporidium infection in domestic geese (Anser anser f. domestica) detected by in-situ hybridization

Journal of Comparative Pathology
S ChvalaHerbert Weissenböck

Abstract

An in-situ hybridization (ISH) procedure was developed for the detection of Cryptosporidium sp. in paraffin wax-embedded tissues with a digoxigenin-labelled probe targeting the 18S rRNA. This technique was used in addition to traditional methods, such as haematoxylin and eosin staining, periodic acid-Schiff reaction, transmission electron microscopy and the polymerase chain reaction, to examine the bursa of Fabricius (BF), conjunctiva and other tissues from 20 domestic geese aged 16-36 days for the presence of cryptosporidia. Positive signals were found to a moderate or marked extent in both conjunctival samples (89%) and BF samples (88%) but not in other tissues. Sequencing of the PCR amplification product revealed identity with Cryptosporidium baileyi. The infected geese showed no clinical signs and only scanty histological lesions. These results confirm reports showing that young waterfowl are especially vulnerable to cryptosporidium infection and indicate that the BF and conjunctiva are the preferred sites for the presence of the protozoon. ISH proved a good method for detecting and identifying even small numbers of cryptosporidia in tissue sections.

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Citations

Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Herbert WeissenböckBarbara Richter
Mar 1, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Meike M MosteglHerbert Weissenböck
Nov 6, 2012·Journal of Comparative Pathology·P A BougiouklisH L Shivaprasad
Aug 8, 2009·Veterinary Parasitology·Judit Plutzer, Panagiotis Karanis
Jun 24, 2009·Experimental Parasitology·Una Ryan
Feb 23, 2008·Veterinary Parasitology·Barbara RichterHerbert Weissenböck
Dec 22, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Ebrahim BadparvaMasoud Badparva
Sep 10, 2013·Journal of Comparative Pathology·B RichterJ Csokai
Oct 9, 2015·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Alex Akira Nakamura, Marcelo Vasconcelos Meireles
Mar 2, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Bettina HabenbacherHerbert Weissenböck
Nov 29, 2007·Microscopy Research and Technique·Barbara RichterHerbert Weissenböck
Jul 3, 2015·BMC Veterinary Research·Julia StadlerAndrea Ladinig
Feb 6, 2017·Journal of Water and Health·Maja Taučer-KapteijnGertjan Medema
Feb 12, 2019·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Maria Cuvertoret-SanzJoaquim Segalés
Jun 18, 2017·Folia Parasitologica·Abd Elkarim LaatamnaMartin Kvac
Oct 8, 2020·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Nicola MeixnerMatthias Müller

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