Cryptosporidium parvum: treatment effects and the rate of decline in oocyst infectivity

The Journal of Parasitology
Angela D CoullietteJoan B Rose

Abstract

Cryptosporidium parvum has become the focus of numerous studies on waterborne disease and transmission in response to outbreaks endangering populations worldwide. The Foci Detection Method-Most Probable Number Assay (FDM-MPN) is an in vitro cell culture method that has been developed and used to determine the quantity of infectious C. parvum oocysts. This research evaluated 2 vendor's producing oocysts, Sterling Parasitology Laboratory (SPL) and Pleasant Hill Farms (PHF) (now known as Bunch Grass Farms as of 12/03), classified as young (<30 days) and aged (>165 days), for comparison of treatments (bleach, antibiotic, no treatment) before cell culture, as well as an age study, to determine any lot-to-lot differences and vendor differences regarding the rate of decline in infectivity. Bleach treatment (0.525%) appeared to be the optimum method for the FDM-MPN with regards to maximum infectivity, efficient disinfection, with no visible antagonistic affects on the C. parvum oocysts. The age study revealed that lot-to-lot variability within each vendor stayed within 1 log10 difference, while the rates of decline in infectivity measured until 107 and 120 days of age when stored at 4 C for SPL and PHF were -0.016 and -0.014 log10 infe...Continue Reading

References

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Citations

Aug 7, 2007·Applied and Environmental Microbiology·Rebecca L IvesJoan B Rose
Apr 25, 2006·The Journal of Parasitology·Barry SteinChristian Chauret
Apr 25, 2006·The Journal of Parasitology·David S LindsayJ P Dubey
Nov 15, 2015·Parasitology Research·Cora DellingMatthias Lendner
Sep 6, 2011·International Journal for Parasitology·P Karanis, H M Aldeyarbi
Aug 5, 2008·Molecular and Biochemical Parasitology·Joann Schmidt, Mark S Kuhlenschmidt

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Software Mentioned

SPSS
MPN
Excel

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