Cryptotanshinone, a compound of Salvia miltiorrhiza inhibits pre-adipocytes differentiation by regulation of adipogenesis-related genes expression via STAT3 signaling

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Naimur RahmanYong-Sik Kim

Abstract

Cryptotanshinone (CT), a major tanshinone found in Salvia miltiorrhiza Bunge (Lamiaceae), has various pharmacological effects such as antitumor, anti-inflammatory, and antioxidant properties. Despite its well-documented benefits in a wide range of diseases, the effect of CT on adipocyte differentiation has not been well characterized. The present study was designed to determine the in vitro anti-adipogenic effect and underlying molecular mechanisms of CT using 3T3-L1 murine pre-adipocytes. We measured the levels of intracellular triglyceride accumulation and mRNA and protein expression of key adipogenic transcription factors and their target genes. Treatment with CT drastically reduced lipid accumulation in a dose- and time-dependent manner. Molecular assays showed that CT effectively suppressed the expression of C/EBPβ, C/EBPα, and PPARγ and of their target adipocyte-specific genes aP2, adiponectin, and GLUT4 but activated the expression of anti-adipogenic genes such as GATA2, CHOP10, and TNF-α. CT treatment also inhibited the phosphorylation of STAT3 in the early phase of adipogenesis. A small-interfering-RNA-mediated knock-down of STAT3 potentiated the anti-adipogenic effect of CT. Taken together, the results suggest that CT...Continue Reading

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Citations

Sep 7, 2016·Biochemical and Biophysical Research Communications·Miso JeonYong-Sik Kim
Nov 4, 2016·Oxidative Medicine and Cellular Longevity·Cheng-Chieh ChangYu-Chiang Hung
Apr 25, 2019·Toxicological Research·Teeranai IttiudomrakChanpen Chanchao
Sep 13, 2017·Scientific Reports·Xi YangBin Wang
Dec 16, 2020·Critical Reviews in Food Science and Nutrition·Han-Ning WangMin Du
Feb 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Huayao LiJibiao Wu
Jul 29, 2018·Chemico-biological Interactions·Seul Gi LeeJu-Ock Nam
Jun 1, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Wenjuan ShenYang Zhang
Apr 30, 2020·Mini Reviews in Medicinal Chemistry·Arturo Estolano-CobiánJoaquín G Marrero

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