PMID: 26343144Sep 8, 2015Paper

Cryptotanshinone Induces Pro-death Autophagy through JNK Signaling Mediated by Reactive Oxygen Species Generation in Lung Cancer Cells

Anti-cancer Agents in Medicinal Chemistry
Wenhui HaoXiuping Chen

Abstract

Cryptotanshinone (CTS), a natural product isolated from Salvia miltiorrhiza Bunge, demonstrates anticancer effect. Previous reports showed that CTS induced caspase-independent cell death. Here, we reported that CTS induced pro-death autophagy in human lung cancer cells. CTS inhibited the proliferation of A549 cells in a time- and concentration- dependent manner. CTS triggered autophagy as confirmed by monodansylcadaverine staining, transmission electron microscopy analysis, as well as western blot detection of microtubule-associated protein light-chain 3 (LC3). CTS induced intracellular reactive oxygen species (ROS) formation in a concentration- and time-dependent manner, which was reversed by N-acetyl-L-cysteine (NAC), catalase, diphenyleneiodonium (DPI), pyrrolinodimethylthiocarbamate (PDTC), and dicumarol. Furthermore, CTS-induced autophagy was inhibited by NAC, JNK siRNA and SP600125. NAC reversed CTS-induced JNK phosphorylation. NAC, 3-methyladenine (3-MA), and SP600125 partly reversed CTS-induced cell death. In addition, CTS (10 mg/kg) dramatically inhibited tumor growth by 48.3% in A549 xenograft nude mice, which was completely reversed by NAC (50 mg/kg) co-treatment. Our findings showed that CTS induced pro-death autoph...Continue Reading

Citations

Jan 14, 2017·The American Journal of Chinese Medicine·Ya-Fang WangJin-Jian Lu
Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yingchun LiangShilin Yang
Apr 12, 2019·Cancer Immunology, Immunotherapy : CII·Shuo LiuJoost J Oppenheim
Apr 28, 2020·BioMed Research International·Hua-Zhong YingWen-Ying Yu
Feb 7, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Huayao LiJibiao Wu

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