Crystal Structure and Potential Head-to-Middle Condensation Function of a Z ,Z -Farnesyl Diphosphate Synthase

ACS Omega
Yueh-Te ChanAndrew H-J Wang

Abstract

Plants produce a wide variety of secondary metabolites in response to adverse environmental factors. Z,Z-Farnesyl diphosphate (Z,Z-FPP), synthesized by Z,Z-farnesyl diphosphate synthase (zFPS), supports the formation of phytochemicals in wild tomatoes. Here, the crystal structure of N-terminal truncated zFPS (ΔzFPS) was determined. Irregular products including lavandulyl diphosphate and an unknown compound were surprisingly found. Apart from the truncated N-terminus as a functional regulator, structure-based analysis and mutagenesis assays revealed a residue H103 in ΔzFPS as one of the key elements to this irregular function. A series of substrate-enzyme complex structures were obtained from ΔzFPS-H103Y by co-crystallizing with isopentenyl diphosphate, dimethylallyl thiolodiphosphate, or both. Various substrate-binding modes were revealed. The catalytic mechanisms of both the head-to-tail and head-to-middle reactions in ΔzFPS were proposed. Functional switch between the two mechanisms in this enzyme and the essential role played by the flexible C-terminus were elucidated as well.

References

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Citations

Dec 5, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Tzu Ping KoYeh Chen
Aug 11, 2020·The Plant Journal : for Cell and Molecular Biology·Garret P MillerBjörn Hamberger
Apr 6, 2019·Acta Crystallographica. Section F, Structural Biology Communications·Tzu Ping KoChun Chi Chen
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Ban H EdaniWilliam C Sessa
Aug 27, 2017·The Journal of Biological Chemistry·Kariona A GrabińskaWilliam C Sessa
Nov 28, 2020·IUBMB Life·Hsin-Yang ChangAndrew H-J Wang
Feb 24, 2020·Current Opinion in Plant Biology·Fei Zhou, Eran Pichersky
Apr 20, 2021·The Journal of Biological Chemistry·Miyako OkadaHisashi Hemmi
Oct 22, 2021·Chembiochem : a European Journal of Chemical Biology·Iryna GerasymenkoHeribert Warzecha

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Coot
Sednterp
CCP4
MOLREP
HKL2000

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