PMID: 8604143Mar 1, 1996Paper

Crystal structure at 2.3 A resolution and revised nucleotide sequence of the thermostable cyclodextrin glycosyltransferase from Thermonanaerobacterium thermosulfurigenes EM1

Journal of Molecular Biology
R M KnegtelB W Dijkstra

Abstract

The crystal structure of the cyclodextrin glycosyltransferase (CGTase) from the thermophilic microorganism Thermoanaerobacterium thermosulfurigenes EM1 has been elucidated at 2.3 A resolution. The final model consists of all 683 amino acid residues, two calcium ions and 343 water molecules, and has a crystallographic R-factor of 17.9% (Rfree 24.9%) with excellent stereochemistry. The overall fold of the enzyme is highly similar to that reported for mesophilic CGTases and differences are observed only at surface loop regions. Closer inspection of these loop regions and comparison with other CGTase structures reveals that especially loops 88-95, 335-339 and 534-539 possibly contribute with novel hydrogen bonds and apolar contacts to the stabilization of the enzyme. Other structural features that might confer thermostability to the T. thermosulfurigenes EM1 CGTase are the introduction of five new salt-bridges and three Gly to Ala/Pro substitutions. The abundance of Ser, Thr and Tyr residues near the active site and oligosaccharide binding sites might explain the increased thermostability of CGTase in the presence of starch, by allowing amylose chains to bind non-specifically to the protein. Additional stabilization of the A/E doma...Continue Reading

Citations

Aug 18, 2009·The FEBS Journal·Camilla ChristiansenBirte Svensson
Apr 25, 2000·Protein Engineering·S KumarR Nussinov
Jan 5, 2005·Applied Microbiology and Biotechnology·Qingsheng Qi, Wolfgang Zimmermann
Mar 1, 2006·Functional Plant Biology : FPB·Crispin A HowittMatthew K Morell
May 20, 2000·Biotechnology & Genetic Engineering Reviews·T Takaha, S M Smith
Sep 20, 2019·Biotechnology Advances·Štefan JanečekBirte Svensson
Jan 1, 1997·Progress in Biophysics and Molecular Biology·S Janecek
Mar 6, 2021·Heliyon·Chin Hui LimMansoor Abdul Hamid
Aug 1, 1997·Current Opinion in Biotechnology·B Lee, G Vasmatzis
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Jan 11, 2001·Biochimica Et Biophysica Acta· van der Veen BAL Dijkhuizen
Jan 16, 2002·Journal of Bacteriology·Naeem RashidTadayuki Imanaka

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