Crystal structure of a novel antifungal protein distinct with five disulfide bridges from Eucommia ulmoides Oliver at an atomic resolution

Journal of Structural Biology
Ye XiangDa-Cheng Wang

Abstract

EAFP2 is a novel antifungal protein isolated from the bark of the tree Eucommia ulmoides Oliver. It consists of 41 residues and is characterized with a five-disulfide motif and the inhibitory effects on the growth of both cell wall chitin-containing and chitin-free fungi. The crystal structure of EAFP2 at an atomic resolution of 0.84 A has been determined by using Shake-and-Bake direct methods with the program SnB. The phases obtained were of sufficient quality to permit the initial model built automatically and the structural refinement carried out using anisotropic displacement parameters resulted in a final crystallographic R factor of 6.8%. In the resulting structural model, all non-hydrogen protein atoms including an unusual pyroglutamyl acid residue at the N-terminal can fit to the articulated electron densities with one centre and more than 65% of the hydrogen atoms in the protein can be observed as individual peaks in the difference map. The general fold of EAFP2 is composed of a 3(10) helix (Cys3-Arg6), an alpha-helix (Ala27-Cys31) and a three-stranded antiparallel beta-sheet (Cys16-Ser18, Cys23-Ser25, and Cys35-Cys37) and cross-linked by five disulfide bridges. The tertiary structure of EAFP2 can be divided into two s...Continue Reading

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Citations

Aug 8, 2012·Acta Crystallographica. Section D, Biological Crystallography·Jeffrey A BellRamy Farid
Nov 16, 2012·Acta Crystallographica. Section D, Biological Crystallography·Dorothee LiebschnerMarcin Olszewski
Mar 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Sergio Trillo-MuyoF Xavier Gomis-Rüth
Nov 19, 2015·Pharmaceuticals·James P TamWei Liang Tan
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May 21, 2014·Journal of Genetics·Dawei WangZhouqi Li
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Jul 2, 2021·Acta Crystallographica. Section A, Foundations and Advances·Jordi Rius, Xavier Torrelles
Jun 10, 2011·Journal of the American Chemical Society·Charles K Fisher, Collin M Stultz

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