Crystal structure of a putative isochorismatase hydrolase from Oleispira antarctica.

Journal of Structural and Functional Genomics
Anna M GoralWladek Minor

Abstract

Isochorismatase-like hydrolases (IHL) constitute a large family of enzymes divided into five structural families (by SCOP). IHLs are crucial for siderophore-mediated ferric iron acquisition by cells. Knowledge of the structural characteristics of these molecules will enhance the understanding of the molecular basis of iron transport, and perhaps resolve which of the mechanisms previously proposed in the literature is the correct one. We determined the crystal structure of the apo-form of a putative isochorismatase hydrolase OaIHL (PDB code: 3LQY) from the antarctic γ-proteobacterium Oleispira antarctica, and did comparative sequential and structural analysis of its closest homologs. The characteristic features of all analyzed structures were identified and discussed. We also docked isochorismate to the determined crystal structure by in silico methods, to highlight the interactions of the active center with the substrate. The putative isochorismate hydrolase OaIHL from O. antarctica possesses the typical catalytic triad for IHL proteins. Its active center resembles those IHLs with a D-K-C catalytic triad, rather than those variants with a D-K-X triad. OaIHL shares some structural and sequential features with other members of th...Continue Reading

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Citations

Sep 17, 2013·Journal of Molecular Biology·Puneet JunejaJennifer N Andexer
Dec 17, 2014·Applied Microbiology and Biotechnology·Jianjun WangSheng Wu
Oct 21, 2016·Applied Microbiology and Biotechnology·Shuaihua GaoGuojun Zheng
Mar 13, 2018·Environmental Microbiology·Serina L RobinsonLawrence P Wackett
Mar 25, 2019·Machine Learning·Ioannis TsamardinosVassilis Christophides
Jul 20, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yang WangLu Yu
Nov 12, 2020·The Journal of Biological Chemistry·Lambros J TassoulasLawrence P Wackett

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