Crystal structure of a Rad51 filament

Nature Structural & Molecular Biology
Adam B ConwayPhoebe A Rice

Abstract

Rad51, the major eukaryotic homologous recombinase, is important for the repair of DNA damage and the maintenance of genomic diversity and stability. The active form of this DNA-dependent ATPase is a helical filament within which the search for homology and strand exchange occurs. Here we present the crystal structure of a Saccharomyces cerevisiae Rad51 filament formed by a gain-of-function mutant. This filament has a longer pitch than that seen in crystals of Rad51's prokaryotic homolog RecA, and places the ATPase site directly at a new interface between protomers. Although the filament exhibits approximate six-fold symmetry, alternate protein-protein interfaces are slightly different, implying that the functional unit of Rad51 within the filament may be a dimer. Additionally, we show that mutation of His352, which lies at this new interface, markedly disrupts DNA binding.

References

Jan 23, 1992·Nature·R M StoryT A Steitz
Jan 23, 1992·Nature·R M Story, T A Steitz
Nov 8, 1996·The Journal of Biological Chemistry·P Sung, S A Stratton
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M M HingoraniS S Patel
Mar 28, 1998·The Journal of Biological Chemistry·Z M YuanD Kufe
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 23, 1999·The Journal of Biological Chemistry·E M ZaitsevaS C Kowalczykowski
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C R KissingerD B Fogel
Apr 23, 1999·The Journal of Biological Chemistry·G ChenE Y Lee
Dec 3, 1999·Progress in Biophysics and Molecular Biology·K D Cowtan, K Y Zhang
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·M D WinnG N Murshudov
Feb 17, 2001·Journal of Molecular Biology·T NiedenzuW Saenger
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·X YuE H Egelman
Feb 13, 2002·Current Opinion in Structural Biology·Jonathan M Caruthers, David B McKay
Nov 21, 2002·Nature·Luca PellegriniAshok R Venkitaraman
Feb 11, 2003·Structure·Margaret S VanLoockEdward H Egelman
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Stephen C West
Oct 7, 2003·Annual Review of Microbiology·Michael M Cox
Oct 8, 2003·Journal of Molecular Biology·Margaret S VanLoockEdward H Egelman
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Aug 13, 2008·Plant Molecular Biology·Chittela RajanikantJayashree K Sainis
Nov 27, 2008·Cell Biochemistry and Biophysics·Magda Budzowska, Roland Kanaar
Sep 11, 2013·Quarterly Reviews of Biophysics·Andrea CandelliGijs J L Wuite
Nov 8, 2006·Biochemistry·Anthony L ForgetKendall L Knight
Mar 3, 2012·Journal of Medicinal Chemistry·Fei HuangAlexander V Mazin
Jan 2, 2008·Cell Research·Xuan Li, Wolf-Dietrich Heyer
Oct 4, 2011·Nature·Karl E DuderstadtJames M Berger
Apr 12, 2013·Nature Communications·Hiroyuki SasanumaAkira Shinohara
Aug 14, 2013·Nature Communications·Yupeng QiuSua Myong
Aug 24, 2006·Nature Reviews. Molecular Cell Biology·Patrick Sung, Hannah Klein
Jan 18, 2007·Nature Reviews. Molecular Cell Biology·Michael M Cox
May 23, 2007·Nature Structural & Molecular Biology·Fumiko EsashiStephen C West
May 23, 2007·Nature Structural & Molecular Biology·Owen Richard Davies, Luca Pellegrini
Apr 19, 2005·Nature Structural & Molecular Biology·Galina V PetukhovaR Daniel Camerini-Otero
Dec 11, 2007·Oncogene·T Thorslund, S C West
Jan 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nayef MazloumWilliam K Holloman
Oct 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Krishanthi JayathilakaPhilip P Connell
Jan 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jovencio HilarioStephen C Kowalczykowski
Nov 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hideyuki ArataGiovanni Cappello
Jul 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anna ReymerBengt Nordén
Apr 3, 2012·The Journal of Biological Chemistry·Yuyong TaoMaikun Teng
Jul 8, 2008·The Journal of Biological Chemistry·Julia M CoxMichael M Cox
Mar 10, 2005·Nucleic Acids Research·Antonio ArizaCharles S Bond
Dec 6, 2005·Nucleic Acids Research·Chris Proudfoot, Richard McCulloch
May 11, 2006·Nucleic Acids Research·Rakhi RajanCharles E Bell
Sep 2, 2006·Nucleic Acids Research·Naoyuki SaraiShigeyuki Yokoyama
Aug 22, 2007·Nucleic Acids Research·Thijn van der HeijdenCees Dekker
Jun 21, 2008·Nucleic Acids Research·Juri HikibaHitoshi Kurumizaka
Nov 27, 2008·Nucleic Acids Research·Xiao-Ping ZhangWolf-Dietrich Heyer
Dec 11, 2008·Nucleic Acids Research·Arabela A GrigorescuPhoebe A Rice
May 19, 2009·Nucleic Acids Research·Yuichi MorozumiHitoshi Kurumizaka
Apr 21, 2010·Nucleic Acids Research·Yoshimasa TakizawaShunichi Takeda
Jan 31, 2012·Nucleic Acids Research·Sara N AndresMurray Junop
Nov 28, 2012·Nucleic Acids Research·Scott AtwellGiovanni Cappello
May 11, 2012·Nucleic Acids Research·Brian BudkePhilip P Connell

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