Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori.

Proteins
Robert LamNickolay Y Chirgadze

Abstract

Urease plays a central role in the pathogenesis of Helicobacter pylori in humans. Maturation of this nickel metalloenzyme in bacteria requires the participation of the accessory proteins UreD (termed UreH in H. pylori), UreF, and UreG, which form sequential complexes with the urease apoprotein as well as UreE, a metallochaperone. Here, we describe the crystal structure of C-terminal truncated UreF from H. pylori (residues 1-233), the first UreF structure to be determined, at 1.55 A resolution using SAD methods. UreF forms a dimer in vitro and adopts an all-helical fold congruent with secondary structure prediction. On the basis of evolutionary conservation analysis, the structure reveals a probable binding surface for interaction with other urease components as well as key conserved residues of potential functional relevance.

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Citations

Nov 10, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Vlad Martin-DiaconescuMichael J Maroney
Jun 26, 2013·Journal of the American Society for Mass Spectrometry·Mark A FarrugiaRobert P Hausinger
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Mar 25, 2011·Journal of Experimental Botany·Joe C PolaccoKristin D Bilyeu
Dec 3, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Barbara ZambelliStefano Ciurli
Sep 10, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Annalisa D'UrzoBarbara Zambelli
May 11, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Francesco BiagiStefano Ciurli
Aug 8, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francesco Musiani, Stefano Ciurli
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Apr 8, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Manfredi MiraulaBarbara Zambelli
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Jul 1, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Matteo MasettiFrancesco Musiani
Mar 30, 2013·The Journal of Biological Chemistry·Mark A FarrugiaRobert P Hausinger
Apr 19, 2021·Seminars in Cancer Biology·Michael J Maroney, Stefano Ciurli

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