Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation

Nature Structural Biology
Jeffrey S KieftJennifer A Doudna

Abstract

The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives internal initiation of viral protein synthesis during host cell infection. In the tertiary structure of the IRES RNA, two helical junctions create recognition sites for direct binding of the 40S ribosomal subunit and eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of the IIIabc four-way junction, which is critical for binding eIF3, reveals how junction nucleotides interact with an adjacent helix to position regions directly involved in eIF3 recognition. Two of the emergent helices stack to form a nearly continuous A-form duplex, while stacking of the other two helices is interrupted by the insertion of junction residues into the helix minor groove. This distorted stack probably serves as an important recognition surface for the translational machinery.

Citations

Jul 5, 2005·Comptes rendus biologies·Andrey V PisarevChristopher U T Hellen
Jan 27, 2004·Journal of Molecular Biology·Sungchul HohngTaekjip Ha
Mar 24, 2004·Trends in Microbiology·Jean-Michel Pawlotsky
Nov 28, 2006·Nature Reviews. Microbiology·Christopher S Fraser, Jennifer A Doudna
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hong JiJennifer A Doudna
Apr 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhouVincent P Mauro
Aug 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Peter V CornishDavid P Giedroc
Jan 10, 2003·Nucleic Acids Research·Sophie BonnalStéphan Vagner
May 9, 2003·Nucleic Acids Research·Thomas LeeperGabriele Varani
Jun 26, 2003·Nucleic Acids Research·Huanwang YangEric Westhof
Sep 16, 2011·Nucleic Acids Research·Christian LaingTamar Schlick
Oct 16, 2012·Nucleic Acids Research·Cristina Romero-LópezAlfredo Berzal-Herranz
May 25, 2007·Journal of Virology·Timothy L TellinghuisenCharles M Rice
Jan 7, 2009·Molecular and Cellular Biology·Keith A SpriggsAnne E Willis
Sep 8, 2006·RNA·Stephen D BairdMartin Holcik
Jul 17, 2009·RNA·Cristina Romero-López, Alfredo Berzal-Herranz
May 18, 2007·RNA·Rahul Tyagi, David H Mathews
Jun 18, 2003·RNA·Sonya E MelcherDavid M J Lilley
Oct 17, 2003·RNA·Olga Fernández-Miragall, Encarnación Martínez-Salas
Sep 1, 2012·PloS One·Helene Minyi LiuMichael M C Lai
Mar 27, 2009·World Journal of Gastroenterology : WJG·Mostafa-K El AwadySaid-A Goueli
Apr 15, 2006·International Journal of Medical Sciences·Volker BrassHubert E Blum
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Larry GoldDom Zichi
Jan 19, 2016·Journal of Molecular Biology·Gloria LozanoEncarnacion Martinez-Salas
Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
May 19, 2009·Journal of Molecular Biology·Christian Laing, Tamar Schlick
Apr 14, 2009·Current Opinion in Structural Biology·Megan E Filbin, Jeffrey S Kieft
May 13, 2008·Trends in Biochemical Sciences·Jeffrey S Kieft
Apr 19, 2008·Trends in Microbiology·Encarnación Martínez-Salas

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

BioHub - Researcher Network

The Chan-Zuckerberg Biohub aims to support the fundamental research and develop the technologies that will enable physicians to cure, prevent, or manage all diseases in our childrens' lifetimes. The CZ Biohub brings together researchers from UC Berkeley, Stanford, and UCSF. Find the latest research from the CZ Biohub researcher network here.

CRISPRi Screens for Antibiotics

The CRISPR-Cas system is a gene editing technique that can be used for high-throughput genome-wide screens to identify modes of actions of novel antibiotics. Here is the latest research.

Biophysics of CRISPR

This feed focuses on broad characteristics of the CRISPR system and the proteins associated with it.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR Genome Editing & Therapy

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of this system for gene editing and therapy in human diseases.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.