Crystal structure of Arabidopsis thaliana NADPH dependent thioredoxin reductase at 2.5 A resolution

Journal of Molecular Biology
S DaiHans Eklund

Abstract

Thioredoxin exists in all organisms and is responsible for the hydrogen transfer to important enzymes for ribonucleotide reduction and the reduction of methionine sulphoxide and sulphate. Thioredoxins have also been shown to regulate enzyme activity in plants and are also involved in the regulation of transcription factors and several other regulatory activities. Thioredoxin is reduced by the flavoenzyme thioredoxin reductase using NADPH. We have now determined the first structure of a eukaryotic thioredoxin reductase, from the plant Arabidopsis thaliana, at 2.5 A resolution. The dimeric A. thaliana thioredoxin reductase is structurally similar to that of the Escherichia coli enzyme, and most differences occur in the loops. Because the plant and E. coli enzymes have the same architecture, with the same dimeric structure and the same position of the redox active disulphide bond, a similar mechanism that involves very large domain rotations is likely for the two enzymes. The subunit is divided into two domains, one that binds FAD and one that binds NADPH. The relative positions of the domains in A. thaliana thioredoxin reductase differ from those of the E. coli reductase. When the FAD domains are superimposed, the NADPH domain of...Continue Reading

References

May 11, 1978·Nature·G E SchulzE F Pai
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jun 1, 1991·European Journal of Biochemistry·P DecottigniesM Miginiac-Maslow
Mar 5, 1990·Journal of Molecular Biology·S K KattiH Eklund
Jun 5, 1987·Journal of Molecular Biology·P A Karplus, G E Schulz
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Oct 20, 1995·Science·C M FraserJ C Venter
Oct 2, 1995·FEBS Letters·P Y GasdaskaG Powis
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·R Rivera-MadridY Meyer
Jan 10, 1994·FEBS Letters·K MinakuchiK Tanaka
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Tamura, T C Stadtman
Oct 15, 1995·Structure·M SaarinenH Eklund
Jan 23, 1987·Science·A T BrüngerM Karplus

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Citations

Jul 27, 2012·The Journal of Biological Chemistry·Pilar Bernal-BayardJosé A Navarro
Apr 26, 2012·Antioxidants & Redox Signaling·Yves MeyerChristophe Riondet
Aug 20, 2009·Acta Crystallographica. Section D, Biological Crystallography·Kristine G KirkensgaardAnette Henriksen
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Marcos Antonio de OliveiraBeatriz Gomes Guimarães
Aug 21, 2009·Annual Review of Genetics·Yves MeyerJean-Philippe Reichheld
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
May 3, 2005·Annual Review of Plant Biology·Bob B Buchanan, Yves Balmer
Jan 29, 2011·BMC Genomics·Ana Karina R AbadioMaria Sueli S Felipe
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Alessia RuggieroLuigi Vitagliano
Oct 15, 2009·Molecular Plant·Juan Manuel Pérez-RuizFrancisco Javier Cejudo
May 4, 2004·Plant Physiology and Biochemistry : PPB·Eric GelhayeJean-Pierre Jacquot
Jan 22, 2008·Biochimica Et Biophysica Acta·Nicolas RouhierJean-Pierre Jacquot
Jan 12, 2005·FEBS Letters·Jean-Philippe ReichheldYves Meyer
Jul 1, 2005·Protein Science : a Publication of the Protein Society·Francis C PetersonBrian F Volkman
May 1, 2001·Current Protocols in Toxicology·E S Arnér, A Holmgren
Dec 18, 2002·Annals of the New York Academy of Sciences·Nicolas RouhierJean-Pierre Jacquot
Nov 4, 2010·The Journal of Microbiology·Hee-Jeong Seo, Young Nam Lee
Dec 7, 2013·The Journal of Biological Chemistry·Lakshmanane PremkumarJennifer L Martin
Sep 30, 2000·European Journal of Biochemistry·C H WilliamsR H Schirmer
Dec 6, 2008·The Journal of Biological Chemistry·Carine DesplatsGilles Peltier
Jun 10, 2004·Physiological Genomics·Jay M EdelbergDongqing Cai
Aug 1, 1997·The New Phytologist·Jean-Pierre JacquotYves Meyer
Sep 28, 1999·Trends in Plant Science·Y MeyerF Vignols
Nov 14, 1998·Biochimica Et Biophysica Acta·V MiskinieneN Cenas
Apr 18, 1998·Biochimica Et Biophysica Acta·D BironaiteN Cenas
Apr 28, 2004·Cytokine & Growth Factor Reviews·Dhananjaya V Kalvakolanu

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