Crystal structure of archaeal chromatin protein Alba2-double-stranded DNA complex from Aeropyrum pernix K1.

The Journal of Biological Chemistry
Tomoyuki TanakaThirumananseri Kumarevel

Abstract

All thermophilic and hyperthermophilic archaea encode homologs of dimeric Alba (Sac10b) proteins that bind cooperatively at high density to DNA. Here, we report the 2.0 Å resolution crystal structure of an Alba2 (Ape10b2)-dsDNA complex from Aeropyrum pernix K1. A rectangular tube-like structure encompassing duplex DNA reveals the positively charged residues in the monomer-monomer interface of each dimer packing on either side of the bound dsDNA in successive minor grooves. The extended hairpin loop connecting strands β3 and β4 undergoes significant conformational changes upon DNA binding to accommodate the other Alba2 dimer during oligomerization. Mutational analysis of key interacting residues confirmed the specificity of Alba2-dsDNA interactions.

References

Apr 20, 1990·European Journal of Biochemistry·A McPherson
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·P J Keeling, W F Doolittle
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·T J Oldfield
Feb 13, 2002·Current Opinion in Structural Biology·Warren L DeLano
Jun 12, 2002·The Journal of Biological Chemistry·Frederic MarcJohn N Reeve
Aug 29, 2002·The EMBO Journal·B N WardleworthM F White
Nov 26, 2002·Trends in Genetics : TIG·Malcolm F White, Stephen D Bell
May 6, 2003·The Journal of Biological Chemistry·Kehao ZhaoRonen Marmorstein
Oct 3, 2003·The Journal of Biological Chemistry·Qiu CuiJinfeng Wang
Nov 25, 2003·Protein Science : a Publication of the Protein Society·Ganggang WangZihe Rao
Dec 4, 2003·Molecular Microbiology·Rong GuoLi Huang
Feb 12, 2004·Methods in Enzymology·Pamela N DyerKarolin Luger
Feb 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrew J ForbesNeil L Kelleher
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 13, 2005·The Journal of Biological Chemistry·Victoria L MarshStephen D Bell
Nov 1, 2005·Current Opinion in Microbiology·Kathleen Sandman, John N Reeve
Nov 16, 2007·Proteins·Thirumananseri KumarevelShigeyuki Yokoyama
Sep 19, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adrien B GeorgesReiner A Veitia
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Dec 29, 2012·Nature Communications·Niels LaurensGijs J L Wuite
Mar 8, 2013·PloS One·Miha ČrnigojNataša Poklar Ulrih
Sep 27, 2014·International Journal of Molecular Sciences·Valeria VisoneMaria Ciaramella
May 31, 2012·Science China. Life Sciences·ZhenFeng ZhangLi Huang
Feb 24, 2016·Biochimica Et Biophysica Acta·Manish GoyalUday Bandyopadhyay
Jan 30, 2013·Biochemical Society Transactions·Rosalie P C Driessen, Remus Th Dame
May 4, 2016·Journal of Bacteriology·Alexandra M GehringThomas J Santangelo
May 7, 2015·Nature Reviews. Microbiology·Eveline PeetersRemus T Dame
Aug 8, 2020·Nucleic Acids Research·Ningning ZhangLi Huang
Apr 16, 2020·Biomolecules·Ping GongHuiqiang Lou
Jan 1, 2021·Journal of Molecular Biology·Shawn P LaursenKarolin Luger
May 15, 2019·Journal of Molecular Biology·Travis J SandersThomas J Santangelo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.