Crystal structure of Cdc11, a septin subunit from Saccharomyces cerevisiae

Journal of Structural Biology
Anton BrausemannThomas Gronemeyer

Abstract

Septins are a conserved family of GTP-binding proteins that assemble into a highly ordered array of filaments at the mother bud neck in Saccharomyces cerevisiae cells. Many molecular functions and mechanisms of the septins in S. cerevisiae were already uncovered. However, structural information is only available from modeling the crystallized subunits of the human septins into the EM cryomicroscopy data of the yeast hetero-octameric septin rod. Octameric rods are the building block of septin filaments in yeast. We present here the first crystal structure of Cdc11, the terminal subunit of the octameric rod and discuss its structure in relation to its human homologues. Size exclusion chromatography analysis revealed that Cdc11 forms homodimers through its C-terminal coiled coil tail.

References

Sep 9, 2000·Biochemistry·A IfflandK Johnsson
May 23, 2002·Bioinformatics·Mauro Delorenzi, Terry Speed
Mar 3, 2004·The Journal of Cell Biology·Matthias Versele, Jeremy Thorner
Jul 20, 2007·Nature·Minhajuddin SirajuddinAlfred Wittinghofer
May 15, 2008·Nature Reviews. Molecular Cell Biology·Christine S WeirichYves Barral
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Aurelie BertinEva Nogales
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Minhajuddin SirajuddinAlfred Wittinghofer
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Alexei Vagin, Alexei Teplyakov
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Aug 10, 2011·Biological Chemistry·Eldar ZentAlfred Wittinghofer
Dec 14, 2011·Molecular Biology of the Cell·Aurélie BertinEva Nogales
Nov 21, 2012·The Biochemical Journal·Joci N A MacedoAna P U Araujo
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov

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Citations

May 26, 2017·ELife·Andrew Weems, Michael McMurray
Aug 10, 2018·Cytoskeleton·Julian David BaurThomas Gronemeyer
Sep 2, 2018·Cytoskeleton·Alexander Mela, Michelle Momany
Jan 9, 2020·Biological Chemistry·Marian Farkašovský
Apr 25, 2020·Journal of Cell Science·Julian CholletNils Johnsson
Jul 30, 2020·The Biochemical Journal·Cyntia TaveneauAurélie Bertin
Sep 15, 2017·Biophysics Reviews·Napoleão Fonseca ValadaresRichard Charles Garratt
May 11, 2018·Cytoskeleton·Megha AbbeyManoj B Menon
Jan 9, 2019·BMC Evolutionary Biology·Benjamin AuxierMichelle Momany
Nov 20, 2016·Frontiers in Cell and Developmental Biology·Oliver Glomb, Thomas Gronemeyer
Jul 7, 2018·Cytoskeleton·Joseph MarquardtErfei Bi
Oct 10, 2020·Nature Communications·Fang JiaoSimon Scheuring
Feb 10, 2021·G3 : Genes - Genomes - Genetics·Julie TakagiGregory C Finnigan

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