Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate.

PloS One
Subramaniam EswaramoorthyA Matin

Abstract

The Escherichia coli ChrR enzyme is an obligatory two-electron quinone reductase that has many applications, such as in chromate bioremediation. Its crystal structure, solved at 2.2 Å resolution, shows that it belongs to the flavodoxin superfamily in which flavin mononucleotide (FMN) is firmly anchored to the protein. ChrR crystallized as a tetramer, and size exclusion chromatography showed that this is the oligomeric form that catalyzes chromate reduction. Within the tetramer, the dimers interact by a pair of two hydrogen bond networks, each involving Tyr128 and Glu146 of one dimer and Arg125 and Tyr85 of the other; the latter extends to one of the redox FMN cofactors. Changes in each of these amino acids enhanced chromate reductase activity of the enzyme, showing that this network is centrally involved in chromate reduction.

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Citations

Sep 10, 2014·Journal of Environmental Management·Hrudayanath ThatoiNigamananda Das
Jan 15, 2018·Environmental Science and Pollution Research International·Paweł KaszyckiWojciech Wesołowski
Feb 4, 2014·Applied and Environmental Microbiology·Rosanne HertzbergerM Joost Teixeira de Mattos
Sep 2, 2018·Protein Science : a Publication of the Protein Society·Jeffrey S McFarlaneHolly R Ellis
Mar 30, 2018·Environmental Science and Pollution Research International·Ornella M OntañonPaola S González
Aug 1, 2020·International Journal of Environmental Research and Public Health·Marina TumoloVito Felice Uricchio
Aug 18, 2020·Applied and Environmental Microbiology·Luz I Valenzuela-GarcíaMario Pedraza-Reyes
Sep 6, 2020·Applied and Environmental Microbiology·Audrey G O'NeillGraham R Moran
Nov 18, 2020·Environmental Microbiology·Huawen HanXiangkai Li
Jun 13, 2021·BMC Microbiology·Sandhya NanjaniHareshkumar Keharia
Nov 28, 2017·Environmental Science & Technology·Isis E Mejias CarpioDebora F Rodrigues

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Methods Mentioned

BETA
size-exclusion chromatography
gel-filtration
size exclusion chromatography

Software Mentioned

wARP
CNS
HKL2000
ARP
ChrR

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