Crystal Structure of Crataeva tapia Bark Protein (CrataBL) and Its Effect in Human Prostate Cancer Cell Lines

PloS One
Rodrigo S FerreiraMaria L V Oliva

Abstract

A protein isolated from the bark of Crataeva tapia (CrataBL) is both a Kunitz-type plant protease inhibitor and a lectin. We have determined the amino acid sequence and three-dimensional structure of CrataBL, as well as characterized its selected biochemical and biological properties. We found two different isoforms of CrataBL isolated from the original source, differing in positions 31 (Pro/Leu); 92 (Ser/Leu); 93 (Ile/Thr); 95 (Arg/Gly) and 97 (Leu/Ser). CrataBL showed relatively weak inhibitory activity against trypsin (Kiapp = 43 µM) and was more potent against Factor Xa (Kiapp = 8.6 µM), but was not active against a number of other proteases. We have confirmed that CrataBL contains two glycosylation sites and forms a dimer at high concentration. The high-resolution crystal structures of two different crystal forms of isoform II verified the β-trefoil fold of CrataBL and have shown the presence of dimers consisting of two almost identical molecules making extensive contacts (∼645 Å(2)). The structure differs from those of the most closely related proteins by the lack of the N-terminal β-hairpin. In experiments aimed at investigating the biological properties of CrataBL, we have shown that addition of 40 µM of the protein for...Continue Reading

References

Oct 1, 1991·Journal of Structural Biology·J van OostrumA Schmitz
Jan 1, 1991·Proteins·E RutenberJ D Robertus
Nov 25, 1988·Nucleic Acids Research·F Corpet
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Apr 1, 1967·Analytical Biochemistry·J R Loewenberg
May 1, 1984·The Journal of Urology·D P De KlerkH J Human
Jul 1, 1984·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J FischerR Fischer
Jun 1, 1980·The Tohoku Journal of Experimental Medicine·M EndoZ Yosizawa
Oct 1, 1995·Plant Physiology·W J Peumans, E J Van Damme
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Aug 28, 1998·Science·D R Green, J C Reed
Oct 16, 1999·The Journal of Biological Chemistry·P C AlmeidaI L Tersariol
Oct 26, 2000·Nature·M O Hengartner
Aug 28, 2002·Anatomia, histologia, embryologia·S Van Cruchten, W Van Den Broeck
Oct 17, 2002·Journal of Agricultural and Food Chemistry·Nathan Sharon, Halina Lis
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Feb 20, 2003·Archives of Biochemistry and Biophysics·M Fernanda TroncosoCarlota Wolfenstein-Todel
Nov 25, 2003·Oncogene·Suzanne CoryJerry M Adams
Feb 21, 2004·CA: a Cancer Journal for Clinicians·Ahmedin JemalUNKNOWN American Cancer Society
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 16, 2005·Analytical Biochemistry·Tatsuya MasukoYuan C Lee
Apr 12, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eiichi SaitohShoji Odani
Sep 27, 2005·Critical Reviews in Food Science and Nutrition·Elvira González De Mejía, Valentin I Prisecaru
May 30, 2006·Biochimica Et Biophysica Acta·Mohamed AzarkanDanielle Baeyens-Volant
Aug 10, 2006·Biological Chemistry·Andrezza J GozzoMariana S Araújo
Dec 14, 2006·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoy
Dec 16, 2006·The Journal of Biological Chemistry·Daisuke HorigomeAkira Uchida

❮ Previous
Next ❯

Citations

Nov 17, 2015·Journal of Agricultural and Food Chemistry·Natalia N S NunesMaria Luiza V Oliva
Aug 8, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Dongwen ZhouAlexander Wlodawer
May 17, 2018·Oncotarget·Camila Ramalho BonturiMaria Luiza Vilela Oliva
Feb 1, 2018·Nanoscale·André L LiraAlioscka A Sousa
Sep 19, 2018·International Journal of Oncology·M J Núñez-IglesiasM Freire-Garabal
Jun 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Camila Ramalho BonturiMaria Luiza Vilela Oliva
Aug 19, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Leydianne Leite de Siqueira PatriotaThiago Henrique Napoleão

❮ Previous
Next ❯

Datasets Mentioned

BETA
B8WI86

Methods Mentioned

BETA
size exclusion chromatography
flow cytometry
ion exchange chromatography
reverse-phase chromatography
ion-exchange chromatography
glycosylation

Software Mentioned

DALI
XDS
Phaser
BLAST
PHENIX
CrataBL
Grafit
REFMAC5
COOT

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Biochemical and Biophysical Research Communications
Lin Eng NgKim Ping Wong
Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Miao WangQiangsong Tong
© 2021 Meta ULC. All rights reserved