Crystal structure of Escherichia coli phosphoenolpyruvate carboxykinase: a new structural family with the P-loop nucleoside triphosphate hydrolase fold

Journal of Molecular Biology
A MatteLouis T J Delbaere

Abstract

The crystal structure of ATP-dependent phosphoenolpyruvate carboxykinase (ATP-oxaloacetate carboxy-lyase, (transphosphorylating), E.C. 4.1.1.49; PCK) from Escherichia coli strain K12 has been determined using a combination of multiple isomorphous replacement, density modification, and partial model phase combination, and refined to a conventional R-index of 0.204 (Rfree = 0.244) at 1.9 A resolution. Each PCK molecule consists of a 275 residue N-terminal domain and 265 residue C-terminal or mononucleotide-binding domain, with the active site postulated to be within a cleft between the two domains. PCK is an open-faced, mixed alpha/beta protein, with each domain having an alpha/beta folding topology as found in several other mononucleoside-binding enzymes. The putative phosphate-binding site of ATP adopts the P-loop motif common to many ATP and GTP-binding proteins, and is similar in structure to that found within adenylate kinase. However, the beta-sheet topology within the mononucleotide-binding fold of PCK differs from all other families within the P-loop containing nucleoside triphosphate hydrolase superfamily, therefore suggesting it represents the first member in a new family of such proteins. The mononucleotide-binding dom...Continue Reading

Citations

Apr 1, 1996·Nature Structural Biology·E F Pai
Dec 24, 1997·Nature Structural Biology·L W TariL T Delbaere
Jul 30, 2009·The Journal of Biological Chemistry·Gerald M Carlson, Todd Holyoak
Feb 13, 2001·Nucleic Acids Research·N V Grishin
Oct 24, 2000·Genome Research·Y WangT Tatusova
Mar 27, 2001·Protein Science : a Publication of the Protein Society·R R CopleyC P Ponting
Jan 1, 1997·Annual Review of Biochemistry·R W Hanson, L Reshef
Jun 2, 2010·The Journal of General and Applied Microbiology·Narito AsanumaTsuneo Hino
Apr 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lei Xie, Philip E Bourne
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jose Antonio MárquezKlaus Scheffzek
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sonia FieulaineSylvie Nessler
Dec 15, 2015·ELife·Vikram AlvaAndrei N Lupas
Jan 26, 1999·Journal of Molecular Biology·G J Kleywegt
Apr 6, 2005·Research in Microbiology·Aaron StonestromMilton H Saier
Dec 20, 2005·Journal of Molecular Biology·Nicola D Gold, Richard M Jackson
Mar 28, 1997·The Journal of Biological Chemistry·A MatteL T Delbaere
Jan 18, 2002·The Journal of Biological Chemistry·Anne GalinierJean-Michel Jault
Mar 30, 2001·The Journal of Biological Chemistry·B MukhopadhyayR S Wolfe
Jan 9, 2020·Biochimica Et Biophysica Acta. General Subjects·Akosiererem SokariboHughes Goldie
Jul 6, 2006·Biochemistry·Todd HolyoakThomas Nowak
Feb 20, 2002·Journal of Molecular Biology·Pete DuntenStanley J Wertheimer
Sep 12, 2001·Journal of Structural Biology·N V Grishin
Oct 1, 1996·Current Opinion in Structural Biology·H S PappaN Q McDonald

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.