Crystal structure of Gib2, a signal-transducing protein scaffold associated with ribosomes in Cryptococcus neoformans

Scientific Reports
Rya EroYong-Gui Gao

Abstract

The atypical Gβ-like/RACK1 Gib2 protein promotes cAMP signalling that plays a central role in regulating the virulence of Cryptococcus neoformans. Gib2 contains a seven-bladed β transducin structure and is emerging as a scaffold protein interconnecting signalling pathways through interactions with various protein partners. Here, we present the crystal structure of Gib2 at a 2.2-Å resolution. The structure allows us to analyse the association between Gib2 and the ribosome, as well as to identify the Gib2 amino acid residues involved in ribosome binding. Our studies not only suggest that Gib2 has a role in protein translation but also present Gib2 as a physical link at the crossroads of various regulatory pathways important for the growth and virulence of C. neoformans.

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Citations

Sep 23, 2016·BioMed Research International·Xue ZhangGuotian Li
Jul 6, 2019·Frontiers in Cellular and Infection Microbiology·Mélissa Caza, James W Kronstad
Apr 30, 2021·Journal of Cell Science·Wenting BuYong-Gui Gao

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Methods Mentioned

BETA
affinity purification
co-immunoprecipitation
X-ray

Software Mentioned

ARP
ray Detector Software ( XDS )
wARP
MacPyMOL
Gib2
COOT
Phenix

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