PMID: 9144156May 13, 1997Paper

Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity

Proceedings of the National Academy of Sciences of the United States of America
M HennigJ N Jansonius


The three-dimensional structure of glutamate-1-semialdehyde aminomutase (EC, an alpha2-dimeric enzyme from Synechococcus, has been determined by x-ray crystallography using heavy atom derivative phasing. The structure, refined at 2.4-A resolution to an R-factor of 18.7% and good stereochemistry, explains many of the enzyme's unusual specificity and functional properties. The overall fold is that of aspartate aminotransferase and related B6 enzymes, but it also has specific features. The structure of the complex with gabaculine, a substrate analogue, shows unexpectedly that the substrate binding site involves residues from the N-terminal domain of the molecule, notably Arg-32. Glu-406 is suitably positioned to repel alpha-carboxylic acids, thereby suggesting a basis for the enzyme's reaction specificity. The subunits show asymmetry in cofactor binding and in the mobilities of the residues 153-181. In the unliganded enzyme, one subunit has the cofactor bound as an aldimine of pyridoxal phosphate with Lys-273 and, in this subunit, residues 153-181 are disordered. In the other subunit in which the cofactor is not covalently bound, residues 153-181 are well defined. Consistent with the crystallographically demonstrated asym...Continue Reading


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Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Apr 1, 1988·Trends in Biochemical Sciences·C G KannangaraD von Wettstein
Jul 1, 1972·Toxicology and Applied Pharmacology·A Hurwitz
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G C FordJ N Jansonius
Apr 15, 1981·Journal of Molecular Biology·C ThallerJ N Jansonius
Sep 15, 1993·European Journal of Biochemistry·A Garnier, R A John
Dec 1, 1996·Current Opinion in Structural Biology·D E Koshland
Oct 1, 1993·Asian American and Pacific Islander Journal of Health·Mia M. Choe
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt, T A Jones

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