PMID: 9437427Jan 23, 1998Paper

Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing

Nature Structural Biology
T KlabundeJ C Sacchettini

Abstract

Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein found in the gyrase A subunit from Mycobacterium xenopi at 2.2 A resolution. The structure contains an unusual beta-fold with the catalytic splice junctions at the ends of two adjacent antiparallel beta-strands. The arrangement of the active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is consistent with a four-step mechanism for the cleavage-ligation reaction. Using site-directed mutagenesis, the N-terminal cysteine, proposed as the nucleophile in the first step of the splicing reaction, was changed to a Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.

References

Oct 15, 1976·Journal of Molecular Biology·G N Ramachandran, A K Mitra
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F B PerlerS K Hempstead
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jun 8, 1968·Nature·J DrenthB G Wolthers
Sep 1, 1995·Trends in Biochemical Sciences·A A Cooper, T H Stevens
Dec 1, 1994·Protein Science : a Publication of the Protein Society·S Pietrokovski
Jan 19, 1995·Nature·H J DugglebyP C Moody
Jan 1, 1994·Methods in Enzymology·A C Storer, R Ménard
Nov 4, 1994·Science·P E DawsonS B Kent
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·H FsihiS T Cole
Feb 23, 1996·The Journal of Biological Chemistry·E R Butch, K L Guan
Dec 1, 1995·Nature Structural Biology·C OinonenL Peltonen
Mar 15, 1997·Nucleic Acids Research·F B PerlerE Adam
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
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Citations

May 8, 2010·Applied Microbiology and Biotechnology·Skander Elleuche, Stefanie Pöggeler
Oct 17, 2002·Journal of Molecular Biology·Marilena Fitzsimons HallIra Schildkraut
Sep 28, 1998·Biochimica Et Biophysica Acta·H WuX Q Liu
Aug 4, 2001·Trends in Genetics : TIG·S Pietrokovski
Sep 7, 2000·Current Opinion in Biotechnology·F B Perler, E Adam
Jul 17, 1998·Current Opinion in Structural Biology·R B Russell, C P Ponting
Apr 8, 2010·Quarterly Reviews of Biophysics·Masatsune Kainosho, Peter Güntert
Jul 28, 2009·Journal of the American Chemical Society·Zhenming DuChunyu Wang
Aug 12, 1998·Nature Structural Biology·M S WeissR Hilgenfeld
May 25, 2010·Nature Chemical Biology·Silvia FrutosTom W Muir
Jan 23, 1998·Nature Structural Biology·B L Stoddard, S Pietrokovski
Apr 18, 1998·Nature Structural Biology·F B Perler
Apr 5, 2011·Nature Structural & Molecular Biology·Brian P CallahanMarlene Belfort
Nov 14, 2006·Nature Structural & Molecular Biology·Linnan ShenRonald T Hay
Dec 6, 2006·Nature Structural & Molecular Biology·Danny T Huang, Brenda A Schulman
Jun 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Gil AmitaiMarlene Belfort
Sep 15, 2011·The Journal of Biological Chemistry·Zhenming DuChunyu Wang
Dec 11, 1999·Nucleic Acids Research·F B Perler
Dec 12, 2012·Protein Engineering, Design & Selection : PEDS·Miguel RamirezZhilei Chen
Sep 17, 2010·Genes & Development·Philip A BeachyChristian Siebold
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Andrzej LyskowskiHideo Iwaï
Feb 28, 2007·Antimicrobial Agents and Chemotherapy·Stéphanie MatratAlexandra Aubry
Jun 21, 2013·Journal of Clinical Microbiology·Tâmara Heloísa Rocha PrandiniEduardo Bagagli
May 23, 2007·Molekuliarnaia biologiia·P L Starokadomskiĭ
Jan 15, 2009·Molekuliarnaia biologiia·P L StarokadomskiĭV A Kordium
May 3, 2005·Annual Review of Plant Biology·Thomas C EvansSriharsa Pradhan
Mar 11, 2003·Annual Review of Biochemistry·Tom W Muir
Nov 13, 2009·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Yasuhiro Anraku, Yoshinori Satow
Mar 29, 2007·International Journal of Biological Sciences·Alireza G Senejani, J Peter Gogarten
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S Rosenblum, G Blobel
Apr 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alessandra RomanelliTom W Muir
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·H WuX Q Liu
May 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Brian P Callahan, Marlene Belfort
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Zhihua LiuTom W Muir
Aug 27, 2013·Nature Chemical Biology·A Sesilja ArankoHideo Iwaï

❮ Previous
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