PMID: 9177170Jun 10, 1997Paper

Crystal structure of heat shock locus V (HslV) from Escherichia coli

Proceedings of the National Academy of Sciences of the United States of America
M BochtlerR Huber

Abstract

Heat shock locus V (HslV; also called ClpQ) is the proteolytic core of the ATP-dependent protease HslVU in Escherichia coli. It has sequence similarity with the beta-type subunits of the eukaryotic and archaebacterial proteasomes. Unlike these particles, which display 72-point symmetry, it is a dimer of hexamers with 62-point symmetry. The crystal structure of HslV at 3.8-A resolution, determined by isomorphous replacement and symmetry averaging, shows that in spite of the different symmetry of the particle, the fold and the contacts between subunits are conserved. A tripeptide aldehyde inhibitor, acetyl-Leu-Leu-norleucinal, binds to the N-terminal threonine residue of HslV, probably as a hemiacetal, relating HslV also functionally to the proteasomes of archaea and eukaryotes.

References

Jan 15, 1992·European Journal of Biochemistry·A L Goldberg
Oct 1, 1990·Acta Crystallographica. Section A, Foundations of Crystallography·L A Tong, M G Rossmann
Nov 1, 1994·Nature Structural Biology·P ZwicklW Baumeister
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M RohrwildA L Goldberg
Aug 1, 1996·Trends in Biochemical Sciences·E C SchirmerS Lindquist

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Citations

Jul 4, 2002·Folia Microbiologica·O Hlavácek, L Váchová
Apr 22, 2005·Archives of Microbiology·Dorte FreesHanne Ingmer
Oct 6, 2006·Journal of Molecular Evolution·Mario X Ruiz-González, Ignacio Marín
Apr 5, 2008·Journal of Molecular Evolution·Ruben E Valas, Philip E Bourne
Mar 24, 2004·Journal of Structural Biology·Istvan BotosAlexander Wlodawer
Dec 17, 2003·Current Opinion in Structural Biology·Michael Groll, Tim Clausen
Apr 8, 2000·Current Opinion in Structural Biology·P ZwicklA Steven
Dec 31, 2002·Current Opinion in Structural Biology·Lucy Vandeputte-Rutten, Piet Gros
Jul 17, 1998·Current Opinion in Structural Biology·A G Murzin
Mar 19, 1999·Trends in Microbiology·R De MotW Baumeister
Nov 14, 1997·Trends in Biochemical Sciences·A LupasW Baumeister
Sep 2, 2003·Metabolic Engineering·Julie A Maupin-FurlowMalgorzata A Gil
Oct 6, 1999·Current Opinion in Chemical Biology·M SchmidtD Finley
May 14, 1999·Current Opinion in Microbiology·S Gottesman
Apr 6, 2004·Nature Structural & Molecular Biology·Shilpa A JoshiRobert T Sauer
Feb 8, 2005·Nature Structural & Molecular Biology·Randall E BurtonRobert T Sauer
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ogura, A J Wilkinson
Jan 29, 2000·Molecular Microbiology·M N PouchW Baumeister
Oct 6, 2009·The Journal of Biological Chemistry·Jung Wook LeeChin Ha Chung
Feb 25, 2009·The Journal of Biological Chemistry·Fredrik I AnderssonAdrian K Clarke
Oct 2, 1998·The EMBO Journal·U Jenal, T Fuchs
Sep 18, 2002·The EMBO Journal·Joaquin OrtegaAlasdair C Steven
Oct 7, 2011·Nucleic Acids Research·Slawomir KubikIgor Konieczny
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P ZwicklW Baumeister
Feb 24, 2001·Protein Science : a Publication of the Protein Society·C Oinonen, J Rouvinen
Aug 12, 2008·Journal of Bacteriology·Lyle A SimmonsGraham C Walker
Aug 10, 2002·Journal of Bacteriology·Shinobu ChibaKoreaki Ito
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M BochtlerR Huber
Apr 5, 2005·Archaea : an International Microbiological Journal·Donald E WardRobert M Kelly
Apr 5, 2005·Archaea : an International Microbiological Journal·B FranzettiG Zaccai
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·H K SongR Huber
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R HoskinsS Wickner
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·H StahlbergC K Suzuki
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·A L HorwichD Finley
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ravishankar RamachandranMatthias Bochtler

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