Crystal structure of Helicobacter pylori pseudaminic acid biosynthesis N-acetyltransferase PseH: implications for substrate specificity and catalysis

PloS One
Abu I Ud-DinA Roujeinikova

Abstract

Helicobacter pylori infection is the common cause of gastroduodenal diseases linked to a higher risk of the development of gastric cancer. Persistent infection requires functional flagella that are heavily glycosylated with 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic acid (pseudaminic acid). Pseudaminic acid biosynthesis protein H (PseH) catalyzes the third step in its biosynthetic pathway, producing UDP-2,4-diacetamido-2,4,6-trideoxy-β-L-altropyranose. It belongs to the GCN5-related N-acetyltransferase (GNAT) superfamily. The crystal structure of the PseH complex with cofactor acetyl-CoA has been determined at 2.3 Å resolution. This is the first crystal structure of the GNAT superfamily member with specificity to UDP-4-amino-4,6-dideoxy-β-L-AltNAc. PseH is a homodimer in the crystal, each subunit of which has a central twisted β-sheet flanked by five α-helices and is structurally homologous to those of other GNAT superfamily enzymes. Interestingly, PseH is more similar to the GNAT enzymes that utilize amino acid sulfamoyl adenosine or protein as a substrate than a different GNAT-superfamily bacterial nucleotide-sugar N-acetyltransferase of the known structure, WecD. Analysis of the complex of PseH with ace...Continue Reading

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Citations

Jan 29, 2016·Biochemistry·Lorenza FavrotOlivia Vergnolle
Sep 16, 2015·Helicobacter·Dionyssios N SgourasYoshio Yamaoka
Jul 2, 2016·International Journal of Molecular Sciences·Abu Iftiaf Md Salah Ud-DinAnna Roujeinikova
Oct 27, 2017·FEBS Letters·Robert GustafssonPål Stenmark
Jan 9, 2020·Organic & Biomolecular Chemistry·Harriet S Chidwick, Martin A Fascione
Apr 17, 2020·Microbiology and Molecular Biology Reviews : MMBR·Rachel M Burckhardt, Jorge C Escalante-Semerena
Oct 29, 2017·Cellular and Molecular Life Sciences : CMLS·Abu Iftiaf Md Salah Ud-Din, Anna Roujeinikova

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Methods Mentioned

BETA
glycosylation
size-exclusion chromatography
PISA

Software Mentioned

MOLPROBITY
iMOSFLM
PHENIX
COOT
Refmac
Autosol
AIMLESS
AREAIMOL
AutoBuild
CCP4

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