PMID: 9417926Jan 7, 1998Paper

Crystal structure of herbicide-detoxifying maize glutathione S-transferase-I in complex with lactoylglutathione: evidence for an induced-fit mechanism

Journal of Molecular Biology
T NeuefeindB Bieseler

Abstract

Glutathione S-transferases (GSTs) -I and -III are involved in herbicide metabolism in maize and have been intensively studied. Starting with plant tissue from Zea mays var. mutin recombinant GST-I was prepared by heterologous expression in Escherichia coli. The enzyme was crystallized in the presence of lactoylglutathione, a ligand formerly never observed in a GST structure and known as an intermediate of the pharmacologically relevant glyoxalase system. The crystal structure of GST-I has been determined at 2.5 A resolution and exhibits the GST-typical dimer of two identical subunits, each consisting of 214 residues. Compared with other plant GSTs the three-dimensional structure of GST-I primarily shows structural differences in the hydrophobic substrate binding site, the linker segment and the C-terminal region. Furthermore, a comparison of the ligand-bound GST-I structure with the apo structure of GST-III indicates the movement of a ten-residue loop upon binding of the ligand to the active site. This is the first structure-based evidence for an induced fit mechanism of glutathione S-transferases, which has previously been postulated for class pi enzymes. Together with GST-III, GST-I may explain herbicide resistance and select...Continue Reading

Citations

Feb 6, 2010·Molecular and Cellular Biochemistry·Soma Banerjee, Riddhi Goswami
Nov 20, 2016·The Journal of Biological Chemistry·Bibi Rafeiza KhanElison B Blancaflor
Oct 18, 2001·Protein Science : a Publication of the Protein Society·A J OakleyM C Wilce
May 4, 2004·Chembiochem : a European Journal of Chemical Biology·Peter Jeschke
Mar 24, 2011·Drug Metabolism Reviews·Ian CumminsRobert Edwards
Jul 27, 2007·DNA Sequence : the Journal of DNA Sequencing and Mapping·Xuefen LiuKexuan Tang
Jan 8, 2015·Frontiers in Plant Science·Henri PégeotNicolas Rouhier
Aug 9, 2007·Marine Biotechnology·Brian BlanchetteBal Ram Singh
Sep 16, 2003·The Journal of Biological Chemistry·Mads Gravers JeppesenGregers Rom Andersen
Aug 25, 2009·Pest Management Science·Peter Jeschke
Apr 10, 2017·Plant Cell Reports·Irini Nianiou-ObeidatNikolaos E Labrou
Jun 14, 2019·Frontiers in Plant Science·Elodie Sylvestre-GononArnaud Hecker
Apr 15, 2003·The Journal of Biological Chemistry·David P DixonRobert Edwards
Oct 13, 2007·Plant Cell Reports·Sang Gon KimKyu Young Kang
Sep 16, 2003·The Journal of Biological Chemistry·Markus PerbandtEva Liebau
Sep 14, 2013·Journal of Biomolecular Structure & Dynamics·Dmitry SuplatovVytas Svedas
Nov 1, 2005·Functional Plant Biology : FPB·Geraldo M A CançadoMarcelo Menossi
Jun 16, 2004·The Biochemical Journal·Irine A AxarliNikolaos E Labrou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.