Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold

Protein Science : a Publication of the Protein Society
Babu A ManjasettyUdo Heinemann

Abstract

The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 A resolution of PTD012 through SAD phasing reveals a monomeric protein and a novel fold. The shorter splice form was also studied and appears to be unfolded and non-functional. The structure of PTD012 displays an alphabetabetaalpha four-layer topology. A metal ion residing between the central beta-sheets is partially coordinated by three histidine residues. X-ray absorption near-edge structure (XANES) analysis identifies the PTD012-bound ion as Zn(2+). Tetrahedral coordination of the ion is completed by the carboxylate oxygen atom of an acetate molecule taken up from the crystallization buffer. The binding of Zn(2+) to PTD012 is reminiscent of zinc-containing enzymes such as carboxypeptidase, carbonic anhydrase, and beta-lactamase. Biochemical assays failed to demonstrate any of these enzyme activities in PTD012. However, PTD012 exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.

Citations

Jul 29, 2010·International Journal of Occupational and Environmental Health·Praneet PensriMontakarn Chaikumarn
May 16, 2009·Journal of Hepatology·Jean Paul BrizardMarie-José Vilarem
Dec 3, 2016·Scientific Reports·Johanna M GostnerFlorian Überall

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