Crystal structure of Legionella pneumophila type IV secretion system effector LegAS4

Biochemical and Biophysical Research Communications
Jonghyeon SonWoo Cheol Lee

Abstract

The SET domain of LegAS4, a type IV secretion system effector of Legionella pneumophila, is a eukaryotic protein motif involved in histone methylation and epigenetic modulation. The SET domain of LegAS4 is involved in the modification of Lys4 of histone H3 (H3K4) in the nucleolus of the host cell, thereby enhancing heterochromatic rDNA transcription. Moreover, LegAS4 contains an ankyrin repeat domain of unknown function at its C-terminal region. Here, we report the crystal structure of LegAS4 in complex with S-adenosyl-l-methionine (SAM). Our data indicate that the ankyrin repeats interact extensively with the SET domain, especially with the SAM-binding amino acids, through conserved residues. Conserved surface analysis marks Glu159, Glu203, and Glu206 on the SET domain serve as candidate residues involved in interaction with the positively charged histone tail. Conserved surface residues on the ankyrin repeat domain surround a small pocket, which is suspected to serve as a binding site for an unknown ligand.

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Citations

Jul 18, 2017·Nature Reviews. Microbiology·Jiazhang Qiu, Zhao-Qing Luo
Nov 28, 2017·Biochemical and Biophysical Research Communications·Guennadi KozlovKalle Gehring
Nov 22, 2017·International Journal of Biological Macromolecules·Zeyaul IslamGhulam Md Ashraf

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