Crystal Structure of Manganese Lipoxygenase of the Rice Blast Fungus Magnaporthe oryzae.

The Journal of Biological Chemistry
Anneli WennmanYang Chen

Abstract

Lipoxygenases (LOX) are non-heme metal enzymes, which oxidize polyunsaturated fatty acids to hydroperoxides. All LOX belong to the same gene family, and they are widely distributed. LOX of animals, plants, and prokaryotes contain iron as the catalytic metal, whereas fungi express LOX with iron or with manganese. Little is known about metal selection by LOX and the adjustment of the redox potentials of their protein-bound catalytic metals. Thirteen three-dimensional structures of animal, plant, and prokaryotic FeLOX are available, but none of MnLOX. The MnLOX of the most important plant pathogen, the rice blast fungusMagnaporthe oryzae(Mo), was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homogeneity, and subjected to crystal screening and x-ray diffraction. The structure was solved by sulfur and manganese single wavelength anomalous dispersion to a resolution of 2.0 Å. The manganese coordinating sphere is similar to iron ligands of coral 8R-LOX and soybean LOX-1 but is not overlapping. The Asn-473 is positioned on a short loop (Asn-Gln-Gly-Glu-Pro) instead of an α-helix and forms hydrogen bonds with Gln-281. Comparison with FeLOX suggests that Phe-332 and Phe-525 might contribute to the unique suprafacia...Continue Reading

References

Aug 20, 1996·Biochemistry·W MinorB Axelrod
May 28, 1998·The Journal of Biological Chemistry·C Su, E H Oliw
Aug 14, 1999·The Journal of Biological Chemistry·A R Brash
Jun 6, 2002·European Journal of Biochemistry·Lena HörnstenErnst H Oliw
Jan 5, 2005·Archives of Biochemistry and Biophysics·Mirela CristeaErnst H Oliw
Jul 7, 2005·Journal of the American Chemical Society·Christian R GoldsmithT Daniel P Stack
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 13, 2010·The Journal of Biological Chemistry·Alexandra AndreouIvo Feussner
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·George M Sheldrick
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jan 15, 2011·Science·Nathaniel C GilbertMarcia E Newcomer
Feb 2, 2011·The FEBS Journal·Florian Brodhun, Ivo Feussner
Sep 23, 2011·Chemical Reviews·Jesper Z Haeggström, Colin D Funk
Apr 5, 2012·Molecular Plant Pathology·Ralph DeanGary D Foster
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
May 11, 2012·The Journal of Biological Chemistry·Priit EekNigulas Samel
Jul 24, 2012·The Journal of Biological Chemistry·Anneli WennmanErnst H Oliw
Mar 15, 2013·Molecular Biology and Evolution·Barry G Hall
Jun 26, 2013·Acta Crystallographica. Section D, Biological Crystallography·Philip R Evans, Garib N Murshudov
Jul 31, 2013·Acta Crystallographica. Section D, Biological Crystallography·James FoadiGwyndaf Evans
Aug 30, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Albert GarretaXavi Carpena
Nov 28, 2013·Applied Microbiology and Biotechnology·Ruud HeshofLeo H de Graaff
Feb 6, 2014·The Journal of Biological Chemistry·Matthew J KobeMarcia E Newcomer
Apr 2, 2014·Chemical Reviews·Yuewei ShengJoan Selverstone Valentine
Aug 28, 2014·The Journal of Biological Chemistry·Andrew W FosterNigel J Robinson
Sep 19, 2014·The Journal of Biological Chemistry·David B NeauMarcia E Newcomer
Dec 20, 2014·Protein Science : a Publication of the Protein Society·Marcia E Newcomer, Alan R Brash

❮ Previous
Next ❯

Citations

Jan 26, 2017·Journal of the American Chemical Society·Masaki HoritaniBrian M Hoffman
Mar 2, 2017·Journal of the American Chemical Society·Hongxin Gao, John T Groves
Mar 25, 2018·Biotechnology and Applied Biochemistry·Yashavanth L VishweshwaraiahBalaji Prakash
Mar 13, 2019·Protein Science : a Publication of the Protein Society·Svetlana PakhomovaMarcia E Newcomer
Oct 21, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Derek B RiceTimothy A Jackson
Jun 25, 2020·Chemical Communications : Chem Comm·Jaycee R MayfieldTimothy A Jackson
May 11, 2017·Essays in Biochemistry·Wen Zhu, Nigel G J Richards
Jul 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Adam R Offenbacher, Theodore R Holman
Dec 2, 2017·The Journal of Biological Chemistry·Adam R OffenbacherJudith P Klinman
Jun 19, 2021·Progress in Lipid Research·Jung-Ung AnDeok-Kun Oh
Oct 9, 2019·Journal of Agricultural and Food Chemistry·Holly StolterfohtHarald Pichler
Sep 26, 2019·Inorganic Chemistry·Amanda W Stubbs, Mircea Dincǎ
Sep 9, 2021·Journal of the American Chemical Society·Adedamola A OpaladeTimothy A Jackson

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2022 Meta ULC. All rights reserved