Crystal structure of Mokola virus glycoprotein in its post-fusion conformation

PLoS Pathogens
Laura BelotAurélie A V Albertini

Abstract

Mokola virus (MOKV) belongs to the lyssavirus genus. As other genus members-including rabies virus (RABV)-it causes deadly encephalitis in mammals. MOKV entry into host cells is mediated by its transmembrane glycoprotein G. First, G binds cellular receptors, triggering virion endocytosis. Then, in the acidic endosomal environment, G undergoes a conformational change from its pre- toward its post-fusion state that catalyzes the merger of the viral and endosomal membranes. Here, we have determined the crystal structure of a soluble MOKV G ectodomain in which the hydrophobic fusion loops have been replaced by more hydrophilic sequences. The crystal structure corresponds to a monomer that is similar to the protomer of the trimeric post-fusion state of vesicular stomatitis virus (VSV) G. However, by electron microscopy, we show that, at low pH, at the surface of pseudotyped VSV, MOKV spikes adopt the trimeric post-fusion conformation and have a tendency to reorganize into regular arrays. Sequence alignment between MOKV G and RABV G allows a precise location of RABV G antigenic sites. Repositioning MOKV G domains on VSV G pre-fusion structure reveals that antigenic sites are located in the most exposed part of the molecule in its pre...Continue Reading

References

Apr 1, 1992·Virology·Y GaudinA Flamand
Aug 1, 1991·Journal of Virology·A BenmansourF Lafay
Dec 1, 1986·Brain Research·T L LentzE Hawrot
Apr 1, 1983·The Journal of General Virology·M LafonR I Macfarlan
Jan 8, 1982·Science·T L LentzG H Tignor
May 1, 1993·Virology·A FlamandR W Ruigrok
Aug 8, 1998·Journal of Virology·M I ThoulouzeM Lafon
Aug 23, 2001·Proceedings of the National Academy of Sciences of the United States of America·N A BakerJ A McCammon
Nov 21, 2001·The Journal of General Virology·C TuffereauP Perrin
Feb 10, 2007·Science·Stéphane RocheStéphane Bressanelli
Dec 25, 2007·Acta Crystallographica. Section A, Foundations of Crystallography·George M Sheldrick
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Oct 6, 2010·The Journal of Cell Biology·Sonia LibersouJean Lepault
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Mar 3, 2012·PLoS Pathogens·Aurélie A AlbertiniYves Gaudin
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Danit FinkelshteinMenachem Rubinstein
Aug 24, 2013·Journal of Virology·Silvia PiccinottiSean P J Whelan
Jan 29, 2016·Methods in Molecular Biology·Marija Backovic, Thomas Krey
Mar 17, 2017·F1000Research·Charles RupprechtFrancois Meslin
Oct 20, 2017·Journal of Virology·Melina VallbrachtMarija Backovic
Mar 14, 2018·Nature Communications·Jovan NikolicAurélie A Albertini

❮ Previous
Next ❯

Citations

Feb 13, 2021·Viruses·Christopher Perry, Andrea C M E Rayat

❮ Previous
Next ❯

Datasets Mentioned

BETA
Y09762.1

Methods Mentioned

BETA
glycosylation
Electron
electron microscopy
electron-microscopy
X-ray
size exclusion chromatography

Software Mentioned

SHELX C / D / E
Pymol
PHASER
PyMOL Molecular Graphics System
PARROT
MOKV
COOT
Buccaneer
PROPKA
Phenix

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.