Crystal structure of periplasmic catecholate-siderophore binding protein VctP from Vibrio cholerae at 1.7 Å resolution

FEBS Letters
Xiuhua LiuLichuan Gu

Abstract

VctP, one of the two essential siderophore-binding PBPs from the pathogen Vibrio cholerae, plays an important role in the transport of enterobactin and vibriobactin, which have quite different configurations of iron coordination, from the periplasm to the inner membrane. The current study reports the crystal structure of VctP from V. cholerae N16961 at 1.7Å resolution. A structural comparison of VctP with its homologues and the results of molecular docking indicate that enterobactin and vibriobactin share the same binding pocket. Significantly, a basic triad consisting of Arg137, Arg226 and Arg270 is used to balance the three negative charges of ferric-enterobactin, while a basic dyad consisting of Arg137 and Arg270 is used to balance the two negative charges of ferric-vibriobactin.

References

May 1, 1992·Journal of Bacteriology·J A StoebnerS M Payne
Jun 27, 1998·Molecular Microbiology·G S Moeck, J W Coulton
Apr 28, 1999·Journal of Bacteriology·S D CarsonP F Sparling
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
Mar 31, 2000·Nature Structural Biology·T E ClarkeL W Tari
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Aug 24, 2006·Angewandte Chemie·Axel MüllerAnne-K Duhme-Klair
Jan 12, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elizabeth E WyckoffShelley M Payne
Sep 7, 2007·Microbiology and Molecular Biology Reviews : MMBR·Marcus Miethke, Mohamed A Marahiel
Apr 29, 2009·Journal of Computational Chemistry·Garrett M MorrisArthur J Olson
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Sep 12, 2009·Angewandte Chemie·Florian PeuckertMohamed A Marahiel
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Anna M ZawadzkaKenneth N Raymond
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Mar 21, 2013·Angewandte Chemie·Daniel J RainesAnne-K Duhme-Klair
Oct 16, 2019·Journal of Bacteriology·Zhenyao LuoChristopher A McDevitt
Jan 30, 2018·Frontiers in Cellular and Infection Microbiology·Carsten JersIvan Mijakovic
Nov 5, 2019·Research in Microbiology·Philippe Delepelaire
May 16, 2017·ACS Infectious Diseases·Nathaniel P EndicottTimothy A Wencewicz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.