Crystal structure of plant light-harvesting complex shows the active, energy-transmitting state.

The EMBO Journal
Tiago BarrosW Kühlbrandt

Abstract

Plants dissipate excess excitation energy as heat by non-photochemical quenching (NPQ). NPQ has been thought to resemble in vitro aggregation quenching of the major antenna complex, light harvesting complex of photosystem II (LHC-II). Both processes are widely believed to involve a conformational change that creates a quenching centre of two neighbouring pigments within the complex. Using recombinant LHC-II lacking the pigments implicated in quenching, we show that they have no particular role. Single crystals of LHC-II emit strong, orientation-dependent fluorescence with an emission maximum at 680 nm. The average lifetime of the main 680 nm crystal emission at 100 K is 1.31 ns, but only 0.39 ns for LHC-II aggregates under identical conditions. The strong emission and comparatively long fluorescence lifetimes of single LHC-II crystals indicate that the complex is unquenched, and that therefore the crystal structure shows the active, energy-transmitting state of LHC-II. We conclude that quenching of excitation energy in the light-harvesting antenna is due to the molecular interaction with external pigments in vitro or other pigment-protein complexes such as PsbS in vivo, and does not require a conformational change within the co...Continue Reading

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Citations

May 17, 2013·Photosynthesis Research·Helmut Kirchhoff
Nov 26, 2013·Chemical Reviews·Rosana ÁlvarezÁngel R de Lera
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stefan BodePeter J Walla
Feb 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·David M Kramer
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Laura WilkWerner Kühlbrandt
Apr 3, 2010·The Journal of Biological Chemistry·Silvia HaferkampHelmut Kirchhoff
Apr 30, 2013·Current Opinion in Structural Biology·Xiaowei PanWenrui Chang
Apr 17, 2012·Journal of Photochemistry and Photobiology. B, Biology·Fu-Gui XiaoLiang Shen
Mar 31, 2015·The Journal of Physical Chemistry. B·Bart van OortIvo H M van Stokkum
Dec 24, 2015·Frontiers in Plant Science·Patrycja HaniewiczDario Piano
Dec 28, 2010·Biochimica Et Biophysica Acta·Luca RondaAndrea Mozzarelli
Aug 25, 2009·Journal of Plant Physiology·Wieslaw I GruszeckiIgnacy Gryczynski
Sep 26, 2015·Journal of Photochemistry and Photobiology. B, Biology·Christopher D P Duffy, Alexander V Ruban
Aug 12, 2014·The Journal of Membrane Biology·Milena OpačićJean-Luc Popot
Mar 31, 2009·Biochimica Et Biophysica Acta·Tiago Barros, Werner Kühlbrandt
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Feb 14, 2013·The Journal of Physical Chemistry. B·Christoph-Peter HolleboomPeter Jomo Walla

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