Crystal structure of soybean 11S globulin: glycinin A3B4 homohexamer

Proceedings of the National Academy of Sciences of the United States of America
Motoyasu AdachiShigeru Utsumi

Abstract

Most plant seeds contain 11S globulins as major storage proteins for their nutrition. Soybean glycinin belongs to the 11S globulin family and consists of five kinds of subunits. We determined the crystal structure of a homohexamer of the glycinin A3B4 subunit at 2.1-A resolution. The crystal structure shows that the hexamer has 32-point group symmetry formed by face-to-face stacking of two trimers. The interface buries the highly conserved interchain disulfide. Based on the structure, we propose that an ingenious face-to-face mechanism controls the hexamer formation of the 11S globulin by movement of a mobile disordered region to the side of the trimer after posttranslational processing. Electrostatic analysis of the faces suggests that the interchain disulfide-containing face has high positive potential at acidic pH, which induces dissociation of the hexamer into trimers that may be susceptible to proteinases after seed imbibition. This dissociation might result in the degradation and mobilization of 11S globulins as storage proteins in embryos during germination and seedling growth.

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Citations

Nov 19, 2009·The Protein Journal·Alejandra QuirogaM Cristina Añón
May 26, 2004·Biochimica Et Biophysica Acta·Tomoyuki Katsube-TanakaShigeru Utsumi
Dec 14, 2012·ACS Applied Materials & Interfaces·Carlos SalasJan Genzer
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