Abstract
Alba (Acetylation lowers binding affinity) domain is a small, dimeric nucleic acid-binding domain, which is widely distributed in archaea and numbers of eukaryotes. Alba domain containing proteins have been reported to be involved in many cellular processes, such as regulation of translation, maintaining genome stability, regulation of RNA processing and so on. In Trypanosoma brucei (T. brucei), there are four Alba proteins identified, which are named TbAlba1 to TbAlba4. However, the structure and function of TbAlba proteins are still unknown. Here, we solved the crystal structure of TbAlba1 to a resolution of 2.46 Å. TbAlba1 adopts a similar Alba-fold, which comprises of four β-strands (β1-β4) and three long α-helices (α1-α3). Furthermore, TbAlba1 displays some structural features quite different from other Alba proteins. These differences may imply the diverse biological roles of Alba family members.
References
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Apr 6, 2002·Science·Stephen D BellMalcolm F White
Aug 29, 2002·The EMBO Journal·B N WardleworthM F White
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Jul 3, 2003·Journal of Bacteriology·Chia-Cheng ChouAndrew H-J Wang
Oct 2, 2003·Genome Biology·L AravindVivek Anantharaman
Jun 25, 2004·Nucleic Acids Research·David E KimDavid Baker
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 31, 2005·Nucleic Acids Research·Ivica LetunicPeer Bork
Jan 24, 2006·Molecular and Biochemical Parasitology·Jennifer B Palenchar, Vivian Bellofatto
Jan 12, 2010·Gene·Lysangela R AlvesSamuel Goldenberg
Aug 4, 2011·PloS One·Jan ManiIsabel Roditi
Oct 4, 2011·Molecular Biology of the Cell·Ines SubotaPhilippe Bastin
Dec 7, 2013·The Journal of Biological Chemistry·Li GuoLi Huang
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Sep 4, 2015·PloS One·Aurélien DupéBarbara Papadopoulou
Dec 15, 2015·Archives of Biochemistry and Biophysics·Chao MaBong-Jin Lee
Feb 24, 2016·Biochimica Et Biophysica Acta·Manish GoyalUday Bandyopadhyay
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Apr 13, 2019·Nucleic Acids Research·Fábio MadeiraRodrigo Lopez
Jan 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jianyi YangDavid Baker