Crystal Structure of Tetrameric Arabidopsis MYC2 Reveals the Mechanism of Enhanced Interaction with DNA

Cell Reports
Teng-Fei LianXiao-Dong Su

Abstract

Jasmonates (JAs) are essential plant hormones that play important roles in the regulation of plant growth and the response to environmental stress. In the JA signaling pathway, the core transcription factors are a class of basic helix-loop-helix (bHLH) proteins, including MYC2, MYC3, and MYC4, that have different regulatory capacities. Here, we report the 2.7 Å crystal structure of the MYC2 bHLH domain complexed with G-box DNA, showing a cis-tetrameric structure. Biochemical assays confirmed that full-length MYC2 forms a stable homo-tetramer both in solution and in DNA-bound states, whereas MYC3 forms only a homodimer. Isothermal titration calorimetry (ITC) assays demonstrated that tetramerization enhanced DNA binding affinity, and fluorescence resonance energy transfer (FRET) assay indicated DNA looping potential of tetrameric MYC2. Luciferase assay further confirmed the importance of tetramerization in transcriptional regulation. Our studies provide a mechanistic explanation for the regulatory differences of MYC transcription factors.

Citations

Mar 15, 2018·Annual Review of Plant Biology·Gregg A HoweAbraham J Koo
Mar 23, 2019·The Plant Journal : for Cell and Molecular Biology·Alicja M GórskaNelson J M Saibo
Dec 27, 2019·Journal of Integrative Plant Biology·Cuiping ZhangJianqiang Wu
Mar 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jose Manuel ChicoRoberto Solano
Oct 6, 2018·Nature Plants·Shohei NosakiMasaru Tanokura
Jul 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Qingqing WuShangwei Zhong
Aug 11, 2019·Nucleic Acids Research·Peter S MillardMeike Burow
Jun 13, 2020·Frontiers in Immunology·Lili Tang, Fusheng Zhou
Jul 27, 2018·Evolutionary Bioinformatics Online·Huang HuangSusheng Song
Dec 15, 2020·Journal of Experimental Botany·Simon Mitreiter, Tamara Gigolashvili
Jan 21, 2021·International Journal of Molecular Sciences·Peter S MillardMeike Burow
Feb 6, 2018·Trends in Plant Science·Maite Colinas, Alain Goossens

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