Crystal structure of the apurinic/apyrimidinic endonuclease IV from Mycobacterium tuberculosis

Biochemical and Biophysical Research Communications
Wei ZhangZihe Rao

Abstract

Endonuclease IV is a typical endonuclease of the apurinic-apyrimidinic (AP) or abasic endonuclease superfamily. It repairs damaged DNA through base excision repair by cleaving the DNA backbone immediately 5' of an AP site. In Mycobacterium tuberculosis, endonuclease IV is the major AP endonuclease. This enzyme is absent from mammalian cells, making it an attractive target for anti-tuberculosis drug development. In this study, the structure of the recombinant endonuclease IV from M. tuberculosis (MtbEndo IV) was determined at a high resolution of 1.18 Å. MtbEndo IV was found to have a classical α8β8-fold TIM barrel with loops on its surface connecting the α-helices and β-strands that constitute a groove for DNA binding. Three zinc ions were identified at the active site. A comparison between the structures of MtbEndo IV and Escherichia coli End IV suggested that Gln32 of MtbEndo IV may plays a role in regulating substrate binding.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Oct 22, 1998·Molecular Microbiology·V Mizrahi, S J Andersen
Sep 3, 2004·Molecular Microbiology·Burkhard SpringerErik C Böttger
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alexander A IshchenkoMurat K Saparbaev
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ivaylo IvanovJ Andrew McCammon
Dec 21, 2007·Acta Crystallographica. Section D, Biological Crystallography·Fei LongGarib N Murshudov
Apr 15, 2008·Nature Structural & Molecular Biology·Elsa D GarcinJohn A Tainer
Jul 4, 2009·Advances in Microbial Physiology·Gregory M CookMichael Niederweis
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Jul 30, 2010·Mutation Research·James M DaleyDindial Ramotar
Mar 2, 2011·Acta Crystallographica. Section D, Biological Crystallography·Ryuichi AsanoRyoji Masui
Mar 5, 2011·Tuberculosis·Pradeep KumarUmesh Varshney
Jul 19, 2011·Tuberculosis·Krishna Kurthkoti, Umesh Varshney
Oct 6, 2011·Archivum Immunologiae Et Therapiae Experimentalis·Carlos K B FerrariAdenilda C Honorio-França
Apr 3, 2012·Traffic·Thierry Soldati, Olivier Neyrolles
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jan 12, 2020·Current Topics in Medicinal Chemistry·Pooja MittalIndrakant K Singh
Mar 21, 2020·Cell Discovery·Yiqing ChenJianhua Gan
Mar 18, 2021·Cell Discovery·Yiqing ChenJianhua Gan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.