Crystal structure of the Bowman-Birk inhibitor from barley seeds in ternary complex with porcine trypsin

Journal of Molecular Biology
Eun Young ParkHyun Kyu Song

Abstract

The structure and function of Bowman-Birk inhibitors (BBIs) from dicotyledonous plants such as soybean have been studied extensively. In contrast, relatively little is known about the BBIs from monocotyledonous plants such as barley, which differ from dicot BBIs in size and tertiary structure. The BBI from barley seeds (BBBI) consists of 125 amino acid residues with two separate inhibitory loops. Previously we determined the high-resolution structure of a 16 kDa BBBI in the free state. The BBBI folds into two compact domains (N and C domain) with tertiary structures that are similar to that of the 8 kDa BBI from dicots. Here we report the structure of a 1:2 complex between BBBI and porcine pancreatic trypsin (PPT) at 2.2 A resolution. This structure confirms that several regions, including the inhibitory loops in the free BBBI structure, show exceptionally low temperature factors and a distorted conformation due to crystalline packing in the lattice. Extensive analysis of the interaction between BBBI and trypsin, and comparison with other known canonical inhibitor-protease complexes, reveals that the mode of interaction between BBBI and PPT is similar to that of known serine protease inhibitors, as expected; however, several un...Continue Reading

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Citations

Nov 15, 2011·Molecules and Cells·Byung-Gil LeeHyun Kyu Song
Oct 15, 2013·Journal of Synchrotron Radiation·Kei FukushimaMidori Takimoto-Kamimura
Dec 18, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Gisele Ferreira EstevesSonia Maria de Freitas
Jan 23, 2016·The New Phytologist·Friederike M Grosse-Holz, Renier A L van der Hoorn
Feb 6, 2014·Rapid Communications in Mass Spectrometry : RCM·Ekaterina DariiJean-Claude Tabet
Jan 25, 2006·Insect Biochemistry and Molecular Biology·A R LopesW R Terra
Nov 17, 2015·Chemistry & Biodiversity·Tibor MaliarJán Kraic
Mar 23, 2010·Nature Structural & Molecular Biology·Byung-Gil LeeHyun Kyu Song
Dec 22, 2010·Journal of Enzyme Inhibition and Medicinal Chemistry·Deepa G Muricken, Lalitha R Gowda
May 10, 2005·Acta Biochimica Et Biophysica Sinica·Rui-Feng QiCheng-Wu Chi

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