Crystal structure of the C-terminal globular domain of oligosaccharyltransferase from Archaeoglobus fulgidus at 1.75 Å resolution

Biochemistry
Shunsuke MatsumotoDaisuke Kohda

Abstract

Protein N-glycosylation occurs in the three domains of life. Oligosaccharyltransferase (OST) transfers glycan to asparagine in the N-glycosylation sequon. The catalytic subunit of OST is called STT3 in eukaryotes, AglB in archaea, and PglB in eubacteria. The genome of a hyperthermophilic archaeon, Archaeoglobus fulgidus, encodes three AglB paralogs. Two of them are the shortest AglBs across all domains of life. We determined the crystal structure of the C-terminal globular domain of the smallest AglB to identify the minimal structural unit. The Archaeoglobus AglB lacked a β-barrel-like structure, which had been found in other AglB and PglB structures. In agreement, the deletion in a larger Pyrococcus AglB confirmed its dispensability for the activity. By contrast, the Archaeoglobus AglB contains a kinked helix bearing a conserved motif, called DK/MI motif. The lysine and isoleucine residues in the motif participate in the Ser/Thr recognition in the sequon. The Archaeoglobus AglB structure revealed that the kinked helix contained an unexpected insertion. A revised sequence alignment based on this finding identified a variant type of the DK motif with the insertion. A mutagenesis study of the Archaeoglobus AglB confirmed the cont...Continue Reading

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Citations

Apr 30, 2013·Nature Chemical Biology·Angelyn LarkinBarbara Imperiali
Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shunsuke MatsumotoDaisuke Kohda
Nov 12, 2013·Applied and Environmental Microbiology·Chen Cohen-RosenzweigJerry Eichler
Apr 10, 2013·Molecular Phylogenetics and Evolution·Lina KaminskiJerry Eichler
Dec 24, 2015·Protein Science : a Publication of the Protein Society·Rei MatsuokaDaisuke Kohda
Sep 4, 2015·Chemical Society Reviews·Dong-Mei LiangJian-Jun Qiao
Jan 30, 2013·Biochemical Society Transactions·Benjamin H Meyer, Sonja-Verena Albers
Jan 30, 2013·Biochemical Society Transactions·Lina KaminskiJerry Eichler
Nov 22, 2012·FEMS Microbiology Reviews·Jerry Eichler, Julie Maupin-Furlow
Sep 23, 2014·Current Opinion in Structural Biology·Tracey M Gloster
Jan 15, 2013·Structure·Zhiyi Wei, Mingjie Zhang
Aug 28, 2016·FEMS Microbiology Reviews·Christina Schäffer, Paul Messner
May 23, 2014·Microbiology and Molecular Biology Reviews : MMBR·Ken F JarrellJerry Eichler
Jan 29, 2013·Nature Reviews. Microbiology·Jerry Eichler
Sep 6, 2020·Molecular Phylogenetics and Evolution·Sophia NikolayevJerry Eichler

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