Crystal structure of the calcium pump with a bound ATP analogue

Nature
Chikashi Toyoshima, Tatsuaki Mizutani

Abstract

P-type ATPases are ATP-powered ion pumps that establish ion concentration gradients across cell and organelle membranes. Here, we describe the crystal structure of the Ca2+ pump of skeletal muscle sarcoplasmic reticulum, a representative member of the P-type ATPase superfamily, with an ATP analogue, a Mg2+ and two Ca2+ ions in the respective binding sites. In this state, the ATP analogue reorganizes the three cytoplasmic domains (A, N and P), which are widely separated without nucleotide, by directly bridging the N and P domains. The structure of the P-domain itself is altered by the binding of the ATP analogue and Mg2+. As a result, the A-domain is tilted so that one of the transmembrane helices moves to lock the cytoplasmic gate of the transmembrane Ca2+-binding sites. This appears to be the mechanism for occluding the bound Ca2+ ions, before releasing them into the lumen of the sarcoplasmic reticulum.

References

Jan 1, 1979·Annual Review of Biochemistry·L de Meis, A L Vianna
Sep 1, 1972·Biochimica Et Biophysica Acta·S Eletr, G Inesi
May 6, 1996·Biochimica Et Biophysica Acta·J V MøllerM le Maire
Jan 16, 1997·Biochimica Et Biophysica Acta·E Mintz, F Guillain
Nov 20, 1997·The Journal of Biological Chemistry·D H MacLennanN M Green
May 19, 1998·Trends in Biochemical Sciences·L AravindE V Koonin
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 29, 2001·Nature Structural Biology·A M BilwesM I Simon
Feb 7, 2002·Journal of Molecular Biology·Chen XuDavid L Stokes
Aug 9, 2002·Nature·Chikashi Toyoshima, Hiromi Nomura
Jan 23, 2003·The Journal of Biological Chemistry·Man Liu, Andreas Barth
May 6, 2003·Nature Structural Biology·Mark HilgeJan Pieter Abrahams
May 17, 2003·The Journal of Biological Chemistry·Hailun MaChikashi Toyoshima
Nov 25, 2003·FEBS Letters·Chikashi ToyoshimaYuji Sugita
Feb 3, 2004·The Journal of Biological Chemistry·Anja Pernille EinholmJens Peter Andersen
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1967·Annual Review of Biochemistry·R W Albers
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Mar 10, 2007·The Journal of Membrane Biology·Ciming LiJean-Daniel Horisberger
Jan 24, 2008·The Journal of Membrane Biology·Hiroshi EguchiKazuo Takeda
Feb 20, 2009·European Biophysics Journal : EBJ·Ronald James Clarke
Oct 15, 2009·European Biophysics Journal : EBJ·Karen McLuskeyNeil W Isaacs
Mar 29, 2012·European Biophysics Journal : EBJ·Anders LervikSigne Kjelstrup
Jul 18, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Yau-Sheng TsaiNobuyo Maeda
May 13, 2010·Journal of Molecular Modeling·Pradeep Kumar NaikHarvinder Singh
Sep 1, 2005·Journal of Industrial Microbiology & Biotechnology·Simon Silver, Le T Phung
May 13, 2006·Journal of Bioenergetics and Biomembranes·Peter L Pedersen
May 13, 2006·Journal of Bioenergetics and Biomembranes·Jesper Vuust MøllerPoul Nissen
Dec 7, 2007·Journal of Bioenergetics and Biomembranes·Anja Pernille EinholmBente Vilsen
Sep 3, 2010·Quarterly Reviews of Biophysics·Jesper V MøllerPoul Nissen
Dec 18, 2007·Biochemistry·Nathaniel J TraasethGianluigi Veglia
Feb 9, 2006·Chemical Reviews·Patrick J O'Brien
Oct 15, 2009·Chemical Reviews·Amie K Boal, Amy C Rosenzweig
Mar 24, 2011·Journal of the American Chemical Society·Mariko MatsunagaJoanna Aizenberg
Nov 19, 2004·Nature·C Roy D Lancaster
Oct 14, 2008·Nature·Ayako TakeuchiDavid C Gadsby
Mar 5, 2013·Nature·Anne-Marie L WintherMorten J Buch-Pedersen
Apr 3, 2009·Nature Reviews. Molecular Cell Biology·David C Gadsby
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·J Preben MorthPoul Nissen
Jun 7, 2005·Nature Structural & Molecular Biology·Anne-Catrin UhlemannSanjeev Krishna
May 31, 2007·Chemical Society Reviews·Xiangyang LiangPeter J Sadler
Aug 16, 2012·Physical Chemistry Chemical Physics : PCCP·Emma S E Eriksson, Leif A Eriksson
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Somshuvra Mukhopadhyay, Adam D Linstedt
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yuji SugitaChikashi Toyoshima
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Shahid MehmoodJean-Michel Jault
Mar 18, 2009·The Journal of Biological Chemistry·Mette LaursenJ Preben Morth
Sep 4, 2009·The Journal of Biological Chemistry·Talya BelogusSteven J D Karlish
Nov 18, 2009·The Journal of Biological Chemistry·Lucia BanciAntonio Rosato
Oct 13, 2010·The Journal of Biological Chemistry·Kazuo YamasakiHiroshi Suzuki
Feb 4, 2011·The Journal of Biological Chemistry·Johannes D ClausenJens Peter Andersen
Apr 2, 2011·The Journal of Biological Chemistry·Irene MangialavoriJuan Pablo F C Rossi
Nov 26, 2011·The Journal of Biological Chemistry·Vivien Rodacker SchackBente Vilsen
Sep 15, 2012·The Journal of Biological Chemistry·Joseph M AutryDavid D Thomas
Mar 20, 2012·The Journal of Biological Chemistry·Leopoldo de Meis
Apr 27, 2005·The Journal of General Physiology·Robert E DempskiErnst Bamberg
Jun 15, 2007·The Journal of General Physiology·R F RakowskiDavid C Gadsby

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