Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p.

Proceedings of the National Academy of Sciences of the United States of America
Jian-Rong SuKunio Miki

Abstract

Pex14p is a central component of the peroxisomal protein import machinery, in which the conserved N-terminal domain mediates dynamic interactions with other peroxins including Pex5p, Pex13p, and Pex19p. Here, we report the crystal structure of the conserved N-terminal domain of Pex14p with a three-helix bundle. A hydrophobic surface is composed of the conserved residues, of which two phenylalanine residues (Phe-35 and Phe-52) protrude to the solvent. Consequently, two putative binding pockets suitable for recognizing the helical WXXXF/Y motif of Pex5p are formed on the surface by the two phenylalanine residues accompanying with positively charged residues. The structural feature agrees well with our earlier findings where F35A/L36A and F52A/L53A mutants were impaired in the interactions with other peroxins such as Pex5p and Pex13p. Pex14p variants each with Phe-to-Ala mutation at positions 35, 52, and 35/52, respectively, were defective in restoring the impaired peroxisomal protein import in pex14 Chinese hamster ovary mutant ZP161 cells. Moreover, in GST pull-down assays His(6)-Pex5pL bound only to GST-Pex14p(25-70), not to any of GST-Pex14p(25-70)F35A, GST-Pex14p(25-70)F52A, and GST-Pex14p(25-70)F35A/F52A. Endogenous Pex5p wa...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·H van den BoschJ M Tager
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S TamuraY Fujiki
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·M FransenS Subramani
Feb 3, 1999·Bioinformatics·J A CuffG J Barton
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P Gallivan, D A Dougherty
Aug 31, 2000·Annual Review of Biochemistry·S SubramaniW B Snyder
Dec 2, 2000·Nature Structural Biology·G J GattoJ M Berg
May 2, 2001·Nature Reviews. Molecular Cell Biology·V I Titorenko, R A Rachubinski
May 4, 2002·Nature Reviews. Molecular Cell Biology·Stephen J Gould, Cynthia S Collins
Dec 19, 2002·Biochimica Et Biophysica Acta·Márcia E M OliveiraJorge E Azevedo
Jan 13, 2004·The Journal of Biological Chemistry·Marc FransenPaul P Van Veldhoven
Jun 25, 2004·Nucleic Acids Research·Burkhard RostJinfeng Liu
Dec 21, 2004·Nucleic Acids Research·Hiroyuki Ogata, Jean-Michel Claverie
Jan 13, 2006·Journal of Molecular Biology·Andreia F CarvalhoJorge E Azevedo
Feb 7, 2006·The Journal of Biological Chemistry·Ryota Itoh, Yukio Fujiki
Oct 19, 2006·Biochimica Et Biophysica Acta·Yukio FujikiKamran Ghaedi
Oct 19, 2006·Biochimica Et Biophysica Acta·Jorge E Azevedo, Wolfgang Schliebs
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Oct 5, 2007·Trends in Cell Biology·Harald W Platta, Ralf Erdmann

❮ Previous
Next ❯

Citations

Jul 7, 2012·Histochemistry and Cell Biology·Carsten TheissRalf Erdmann
Jun 30, 2009·Biological Chemistry·Wolfgang GirzalskyRalf Erdmann
Sep 18, 2012·Cellular and Molecular Life Sciences : CMLS·Harald W PlattaRalf Erdmann
Feb 12, 2010·Biology of the Cell·Thomas Lanyon-HoggAlison Baker
Oct 10, 2015·Biochimica Et Biophysica Acta·Leonidas EmmanouilidisMichael Sattler
Nov 2, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Li JiangYukio Fujiki
Sep 27, 2012·The Biochemical Journal·Ryuichi NatsuyamaYukio Fujiki
May 24, 2012·Biochimica Et Biophysica Acta·Shirisha NagotuHarald W Platta
Mar 3, 2010·Biochemical and Biophysical Research Communications·Jian-Rong SuKunio Miki
Nov 16, 2013·The Journal of Biological Chemistry·Alexander NeuhausRalf Erdmann
Feb 15, 2011·The EMBO Journal·Madhav SukumaranIngo H Greger
Aug 28, 2020·The Journal of Cell Biology·Koichiro YamashitaYukio Fujiki
Jun 11, 2016·Biochemical Society Transactions·Alison BakerStuart L Warriner
Dec 17, 2020·Proceedings of the National Academy of Sciences of the United States of America·Pascal LillChristos Gatsogiannis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.