Crystal structure of the dimeric C-terminal domain of TonB reveals a novel fold

The Journal of Biological Chemistry
C ChangA Wlodawer

Abstract

The TonB-dependent complex of Gram-negative bacteria couples the inner membrane proton motive force to the active transport of iron.siderophore and vitamin B(12) across the outer membrane. The structural basis of that process has not been described so far in full detail. The crystal structure of the C-terminal domain of TonB from Escherichia coli has now been solved by multiwavelength anomalous diffraction and refined at 1.55-A resolution, providing the first evidence that this region of TonB (residues 164-239) dimerizes. Moreover, the structure shows a novel architecture that has no structural homologs among any known proteins. The dimer of the C-terminal domain of TonB is cylinder-shaped with a length of 65 A and a diameter of 25 A. Each monomer contains three beta strands and a single alpha helix. The two monomers are intertwined with each other, and all six beta-strands of the dimer make a large antiparallel beta-sheet. We propose a plausible model of binding of TonB to FhuA and FepA, two TonB-dependent outer-membrane receptors.

References

Nov 16, 1992·Science·H Nikaido, M H Saier
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S M NewtonP E Klebba
Jun 6, 1998·Journal of Structural Biology·T Schirmer
Sep 2, 1998·Molecular Microbiology·M EhrmannA Schlösser
Sep 28, 1998·Journal of Bacteriology·C J LazdunskiH Bénédetti
Oct 8, 1998·Acta Crystallographica. Section D, Biological Crystallography·K Cowtan, P Main
Jan 14, 1999·Nature Structural Biology·S K BuchananJ Deisenhofer
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·R M Esnouf
Apr 21, 1999·Molecular Microbiology·R A LarsenK Postle
May 20, 1999·Nature Structural Biology·A PerrakisV S Lamzin
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·N Cadieux, R J Kadner
Feb 10, 2000·Acta Crystallographica. Section D, Biological Crystallography·Z DauterK R Rajashankar
Mar 4, 2000·Nature Structural Biology·H J MerianosD S Cafiso
Feb 13, 2001·Trends in Biochemical Sciences·S K Buchanan
Feb 15, 2001·Acta Crystallographica. Section D, Biological Crystallography·Z DauterA Wlodawer
Mar 30, 2001·The Journal of Biological Chemistry·D C ScottP E Klebba
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen

❮ Previous
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Citations

May 17, 2005·Journal of Industrial Microbiology & Biotechnology·Raquel QuatriniDavid S Holmes
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·R Sean PeacockHans J Vogel
Nov 9, 2002·Biochimie·Zhenghua Cao, Phillip E Klebba
Aug 16, 2001·Trends in Biochemical Sciences·P Bayer, S Harjes
Apr 6, 2002·Current Opinion in Microbiology·Volkmar Braun, Michael Braun
Feb 4, 2003·Nature Reviews. Molecular Cell Biology·José D Faraldo-Gómez, Mark S P Sansom
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lorne D JordanPhillip E Klebba
Mar 26, 2010·The Journal of Biological Chemistry·Salete M NewtonPhillip E Klebba
Dec 23, 2004·Molecular Microbiology·Joydeep Ghosh, Kathleen Postle
Jan 27, 2004·Infection and Immunity·Amanda J BeddekPaul R Langford
Apr 12, 2012·Journal of Bacteriology·Anne A Ollis, Kathleen Postle
Apr 9, 2008·Journal of Bacteriology·Wallace A KasererPhillip E Klebba
Aug 4, 2006·Journal of Bacteriology·Hendrik AdamsHervé Celia
Aug 24, 2002·Journal of Bacteriology·Kiara G Held, Kathleen Postle
Oct 26, 2002·Journal of Bacteriology·Christopher D Kenney, Cynthia Nau Cornelissen
Mar 2, 2002·Journal of Bacteriology·Qixun Zhao, Keith Poole
Mar 2, 2002·Journal of Bacteriology·Penelope I HiggsKathleen Postle
Sep 18, 2003·Journal of Bacteriology·Annette SauterVolkmar Braun
Feb 4, 2003·Journal of Bacteriology·Alexandra R Mey, Shelley M Payne
Dec 24, 2010·MBio·Kathleen PostleCheryl D Swayne
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Apr 2, 2011·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Karla D Krewulak, Hans J Vogel
Mar 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laurent TerradotGabriel Waksman
Jun 3, 2006·Science·Peter D PawelekJames W Coulton
Dec 23, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ranjan ChakrabortyDick van der Helm
Jan 17, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Byron C H ChuHans J Vogel
Jan 18, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Kathleen Postle, Ray A Larsen
Apr 24, 2007·Biophysical Journal·James GumbartEmad Tajkhorshid
Aug 25, 2015·Protein Expression and Purification·Fernando GuerreroHideo Iwaï
Apr 2, 2005·Protein Science : a Publication of the Protein Society·Cezar M KhursigaraJames W Coulton
Jul 25, 2009·Molecular Microbiology·Anne A OllisKathleen Postle
Aug 2, 2003·Molecular Microbiology·Kathleen Postle, Robert J Kadner
Jun 26, 2003·Molecular Microbiology·Ray A LarsenKathleen Postle
Nov 9, 2011·Environmental Microbiology·Mara StevanovicEnrico Schleiff
Jan 13, 2005·Journal of Molecular Biology·R Sean PeacockHans J Vogel

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