Crystal structure of the GerBC component of a Bacillus subtilis spore germinant receptor.

Journal of Molecular Biology
Yunfeng LiBing Hao

Abstract

The nutrient germinant receptors (nGRs) of spores of Bacillus species are clusters of three proteins that play a critical role in triggering the germination of dormant spores in response to specific nutrient molecules. Here, we report the crystal structure of the C protein of the GerB germinant receptor, so-called GerBC, of Bacillus subtilis spores at 2.3 A resolution. The GerBC protein adopts a previously uncharacterized type of protein fold consisting of three distinct domains, each of which is centered by a beta sheet surrounded by multiple alpha helices. Secondary-structure prediction and structure-based sequence alignment suggest that the GerBC structure represents the prototype for C subunits of nGRs from spores of all Bacillales and Clostridiales species and defines two highly conserved structural regions in this family of proteins. GerBC forms an interlocked dimer in the crystalline state but is predominantly monomeric in solution, pointing to the possibility that GerBC oligomerizes as a result of either high local protein concentrations or interaction with other nGR proteins in spores. Our findings provide the first structural view of the nGR subunits and a molecular framework for understanding the architecture, conser...Continue Reading

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Citations

Dec 20, 2011·Journal of Bacteriology·Mary J WilsonPhilip C Hanna
Apr 24, 2014·BMC Microbiology·Elisabeth H MadslienToril Lindbäck
Feb 18, 2014·Journal of Molecular Biology·Yunfeng LiBing Hao
Nov 30, 2010·Trends in Microbiology·Daniel Paredes-SabjaMahfuzur R Sarker
Nov 28, 2014·The Journal of Antimicrobial Chemotherapy·Mark J LeggettJean-Yves Maillard
Feb 4, 2014·Journal of Bacteriology·Peter Setlow
Jul 13, 2017·Annual Review of Microbiology·Peter SetlowYong-Qing Li
May 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yunfeng LiBing Hao
Jun 24, 2016·Microbiology Spectrum·Anne Moir, Gareth Cooper

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