Crystal structure of the HP1-EMSY complex reveals an unusual mode of HP1 binding

Structure
Ying HuangRui-Ming Xu

Abstract

Heterochromatin protein-1 (HP1) plays an essential role in both the assembly of higher-order chromatin structure and epigenetic inheritance. The C-terminal chromo shadow domain (CSD) of HP1 is responsible for homodimerization and interaction with a number of chromatin-associated nonhistone proteins, including EMSY, which is a BRCA2-interacting protein that has been implicated in the development of breast and ovarian cancer. We have determined the crystal structure of the HP1beta CSD in complex with the N-terminal domain of EMSY at 1.8 A resolution. Surprisingly, the structure reveals that EMSY is bound by two HP1 CSD homodimers, and the binding sequences differ from the consensus HP1 binding motif PXVXL. This structural information expands our understanding of HP1 binding specificity and provides insights into interactions between HP1 homodimers that are likely to be important for heterochromatin formation.

References

Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R Paro, D S Hogness
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J C EissenbergS C Elgin
Jun 1, 1989·Molecular & General Genetics : MGG·G WustmannG Reuter
Aug 25, 1995·Nucleic Acids Research·R Aasland, A F Stewart
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Feb 5, 2000·Current Biology : CB·J F Smothers, S Henikoff
Apr 8, 2000·Current Opinion in Genetics & Development·J C Eissenberg, S C Elgin
Mar 22, 2001·Molecular and Cellular Biology·J F Smothers, S Henikoff
Aug 3, 2001·Nature·S J NielsenT Kouzarides
Aug 11, 2001·Science·T Jenuwein, C D Allis
Dec 26, 2001·The Journal of Biological Chemistry·Stéphane Ansieau, Achim Leutz
Feb 23, 2002·Science·Steven A Jacobs, Sepideh Khorasanizadeh
Mar 15, 2002·Current Opinion in Genetics & Development·Shiv I S Grewal, Sarah C R Elgin
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Milo F Vassallo, Naoko Tanese
Aug 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yuhong LiLori L Wallrath
Apr 5, 2003·Annual Review of Biochemistry·Laura N RuscheJasper Rine
May 2, 2003·Genetica·Mohammed M ShareefRebecca Kellum
Dec 4, 2003·Cell·Luke Hughes-DaviesTony Kouzarides
Apr 9, 2004·Nature Reviews. Molecular Cell Biology·Christèle Maison, Geneviève Almouzni
Sep 30, 2004·Journal of Cell Science·Maria Rosa LidonniciAlessandra Montecucco
Mar 31, 2005·Current Opinion in Genetics & Development·Dmitri A Nusinow, Barbara Panning
May 11, 2005·Biochemical and Biophysical Research Communications·Mark S LechnerFrank J Rauscher
Jun 11, 2005·EMBO Reports·Caroline M S EkbladLaura S Itzhaki
Jun 28, 2005·Journal of Molecular Biology·Gayatri B ChavaliAidan J Doherty
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Jul 1, 2008·Experimental & Molecular Medicine·Pil-Gu Park, Hyunsook Lee
Mar 4, 2011·BMC Evolutionary Biology·Nicholas J Brideau, Daniel A Barbash
Oct 17, 2015·Expert Review of Gastroenterology & Hepatology·Antonella CianferoniAmanda Muir
Oct 26, 2011·Critical Reviews in Biochemistry and Molecular Biology·Bartlomiej J BlusSepideh Khorasanizadeh
Oct 7, 2010·Critical Reviews in Biochemistry and Molecular Biology·Kyoko L Yap, Ming-Ming Zhou
Nov 11, 2015·Best Practice & Research. Clinical Gastroenterology·Patrick M A SleimanHakon Hakonarson
Sep 4, 2013·Angewandte Chemie·Nasrollah Rezaei-GhalehMarkus Zweckstetter
Apr 8, 2011·Chembiochem : a European Journal of Chemical Biology·Deanna L MendezSarah C R Elgin
Mar 13, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing HouGong Yang
Oct 20, 2009·Trends in Biochemical Sciences·Mustafa BillurPrim B Singh
Nov 13, 2012·Journal of Molecular Biology·Yuichi MishimaIsao Suetake
Apr 16, 2013·PloS One·Francesca MunariMarkus Zweckstetter
Aug 21, 2019·Life Science Alliance·Giulia FontiMatteo Dal Peraro
May 1, 2018·Oncotarget·Agnieszka Dansonka-MieszkowskaJolanta Kupryjanczyk
Apr 12, 2012·The Journal of Biological Chemistry·Alexandria N RichartJean O Thomas
Apr 8, 2020·Royal Society Open Science·Prim B Singh, Andrew G Newman
Jan 12, 2011·The Journal of Biological Chemistry·Xingfu XuWilliam A Horne

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