Crystal Structure of the Minor Pilin CofB, the Initiator of CFA/III Pilus Assembly in Enterotoxigenic Escherichia coli.

The Journal of Biological Chemistry
Subramania KolappanLisa Craig

Abstract

Type IV pili are extracellular polymers of the major pilin subunit. These subunits are held together in the pilus filament by hydrophobic interactions among their N-terminal α-helices, which also anchor the pilin subunits in the inner membrane prior to pilus assembly. Type IV pilus assembly involves a conserved group of proteins that span the envelope of Gram-negative bacteria. Among these is a set of minor pilins, so named because they share their hydrophobic N-terminal polymerization/membrane anchor segment with the major pilins but are much less abundant. Minor pilins influence pilus assembly and retraction, but their precise functions are not well defined. The Type IV pilus systems of enterotoxigenic Escherichia coli and Vibrio cholerae are among the simplest of Type IV pilus systems and possess only a single minor pilin. Here we show that the enterotoxigenic E. coli minor pilins CofB and LngB are required for assembly of their respective Type IV pili, CFA/III and Longus. Low levels of the minor pilins are optimal for pilus assembly, and CofB can be detected in the pilus fraction. We solved the 2.0 Å crystal structure of N-terminally truncated CofB, revealing a pilin-like protein with an extended C-terminal region composed ...Continue Reading

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Citations

Aug 19, 2016·Frontiers in Microbiology·Zeus Saldaña-AhuactziJuan Xicohtencatl-Cortes
Dec 4, 2016·Biochemical Society Transactions·Kurt H Piepenbrink, Eric J Sundberg
Jun 27, 2018·Proceedings of the National Academy of Sciences of the United States of America·Hiroya OkiShota Nakamura
Feb 26, 2019·Frontiers in Molecular Biosciences·Kurt H Piepenbrink
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Dec 1, 2019·Medical Microbiology and Immunology·Theis JacobsenMichael Nilges
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