Crystal structure of the MSMEG_4306 gene product from Mycobacterium smegmatis

Acta Crystallographica. Section F, Structural Biology Communications
Adarsh Kumar, Subramanian Karthikeyan

Abstract

The MSMEG_4306 gene from Mycobacterium smegmatis encodes a protein of unknown function with 242 amino-acid residues that contains a conserved zinc-ribbon domain at its C-terminus. Here, the crystal structure of MSMEG_4306 determined by the single-wavelength anomalous dispersion method using just one zinc ion co-purified with the protein is reported. The crystal structure of MSMEG_4306 shows a coiled-coil helix domain in the N-terminal region and a zinc-ribbon domain in the C-terminal region. A structural similarity search against the Protein Data Bank using MSMEG_4306 as a query revealed two similar structures, namely CT398 from Chlamydia trachomatis and HP0958 from Helicobacter pylori, although they share only ∼15% sequence identity with MSMEG_4306. Based on comparative analysis, it is predicted that MSMEG_4306 may be involved in secretion systems, possibly by interacting with multiple proteins or nucleic acids.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Feb 9, 2000·Trends in Microbiology·N BouhoucheC I Kado
Feb 24, 2001·Nature·J C RainP Legrain
Mar 27, 2003·Molecular Microbiology·Christopher M SassettiEric J Rubin
Apr 1, 2003·IUBMB Life·Jacqueline M Matthews, Margaret Sunde
Feb 5, 2004·Chembiochem : a European Journal of Chemical Biology·Jody M Mason, Katja M Arndt
Feb 6, 2004·Journal of Bacteriology·Riccardo ManganelliIssar Smith
Jul 1, 1996·Acta Crystallographica. Section D, Biological Crystallography·G J Kleywegt
Nov 5, 2004·Molecular Biology of the Cell·Kwasi G MawuenyegaXian Chen
Apr 27, 2005·Proteins·Chaya S Rapp, Rena M Pollack
Jun 11, 2005·FEMS Microbiology Letters·Kieran A RyanPaul W O'Toole
Jun 22, 2005·Journal of Bacteriology·Lara Pereira, Timothy R Hoover
Jul 12, 2005·Journal of Molecular Biology·Eran EyalVladimir Sobolev
Oct 27, 2006·FEMS Microbiology Reviews·Sébastien RodrigueRiccardo Manganelli
Mar 21, 2007·Genome Biology·Birgit EisenhaberMarie-Theres Hauser
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Aug 30, 2008·The International Journal of Biochemistry & Cell Biology·M Andrea Markus, Brian J Morris
Oct 22, 2008·Journal of Bacteriology·Francois P DouillardPaul W O'Toole
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Dec 17, 2009·The Biochemical Journal·Andrew D HansonValérie de Crécy-Lagard
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 12, 2010·Nucleic Acids Research·Liisa Holm, Päivi Rosenström
Nov 16, 2010·Future Microbiology·Meghan E FeltcherMiriam Braunstein
Feb 26, 2011·Omics : a Journal of Integrative Biology·Daniel J Rigden
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Aug 27, 2011·Journal of Molecular Modeling·Marbella Maria da FonsêcaAna Tereza Ribeiro Vasconcelos
Sep 17, 2011·Journal of Proteomics·Gustavo A de SouzaHarald G Wiker
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Apr 19, 2012·Biology Direct·Grzegorz M BoratynThomas L Madden
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Nov 16, 2013·Nature Communications·Pavol Skubák, Navraj S Pannu
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Jul 16, 2015·Protein Science : a Publication of the Protein Society·Michael L BartaP Scott Hefty
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Dec 3, 2016·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant

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Datasets Mentioned

BETA
SGC59090

Methods Mentioned

BETA
PCR
size-exclusion chromatography
X-ray
PISA
gel-filtration

Software Mentioned

REFMAC
DALI
MAFFT
CTRUNCATE
CCP
PISA ( Protein Interfaces , Surfaces and Assemblies analysis )
BLAST
Coot
phenix
ESPript

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