Crystal structure of the receptor-binding domain of alpha 2-macroglobulin

Structure
Lasse JennerJens Nyborg

Abstract

The large plasma proteinase inhibitors of the alpha 2-macroglobulin superfamily inhibit proteinases by capturing them within a central cavity of the inhibitor molecule. After reaction with the proteinase, the alpha-macroglobulin-proteinase complex binds to the alpha-macroglobulin receptor, present in the liver and other tissues, and becomes endocytosed and rapidly removed from the circulation. The complex binds to the receptor via recognition sites located on a separate domain of approximately 138 residues positioned at the C terminus of the alpha-macroglobulin subunit. The crystal structure of the receptor-binding domain of bovine alpha 2-macroglobulin (bRBD) has been determined at a resolution of 1.9 A. The domain primarily comprises a nine-strand beta structure with a jelly-roll topology, but also contains two small alpha helices. The surface patch responsible for receptor recognition is thought to involve residues located on one of the two alpha helices of the bRBD as well as residues in two of the beta strands. Located on this alpha helix are two lysine residues that are important for receptor binding. The structure of bRBD is very similar to the approximately 100-residue C-terminal domain of factor XIII, a transglutaminas...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G BuA L Schwartz
May 21, 1992·Nature·S ChoeD Eisenberg
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Aug 31, 1987·FEBS Letters·J Gliemann, L Sottrup-Jensen
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C C KanG Fey
Jan 1, 1985·Annual Review of Cell Biology·J L GoldsteinW J Schneider
Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·K L NielsenM Etzerodt
Oct 20, 1995·The Journal of Biological Chemistry·G R AndersenJ Nyborg
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·N L DalyR Smith
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·V C YeeD C Teller
Sep 5, 1993·Journal of Molecular Biology·L Holm, C Sander
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·P R NielsenF M Poulsen
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Nov 23, 1978·Nature·S C HarrisonG Bricogne

❮ Previous
Next ❯

Citations

Sep 18, 2002·Immunological Reviews·M Amin Arnaout
Jun 24, 2000·European Journal of Biochemistry·M GunnarssonP E Jensen
Dec 6, 2000·Protein Science : a Publication of the Protein Society·T XiaoS R Sprang
Jul 21, 2010·Alzheimer's Research & Therapy·Kerensa BroersenJoost Schymkowitz
Sep 19, 2008·PloS One·Yuji YoshiikeAkihiko Takashima
Sep 16, 2014·Nature Communications·Steve G Wong, Andréa Dessen
Sep 5, 2007·Molecular Immunology·Gérard J ArlaudSthanam V L Narayana
Dec 22, 2004·The Prostate·Victor K LinClaus G Roehrborn
Feb 1, 2012·Angewandte Chemie·Aniebrys MarreroF Xavier Gomis-Rüth
Apr 17, 2009·Structure·Vengadesan KrishnanSthanam V L Narayana
Jul 31, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Fengrong WangKestutis Bendinskas
Aug 1, 2006·Molecular Immunology·Bert J C Janssen, Piet Gros
Jul 3, 2007·Nature Structural & Molecular Biology·Kevin D CorbettJames M Berger
Mar 3, 2017·Biological Chemistry·Theodoros GoulasF Xavier Gomis-Rüth
Dec 1, 2014·Biophysics Reviews·Marni Williams, Richard Baxter
Jan 8, 2000·The Journal of Biological Chemistry·W HuangP G Gettins
Jul 13, 2006·Journal of Molecular Biology·Folmer FredslundLars Sottrup-Jensen
Aug 30, 2006·Journal of Molecular Biology·Gitte A JensenBirthe B Kragelund

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.