PMID: 8942978Nov 26, 1996Paper

Crystal structure of the secretory form of membrane-associated human carbonic anhydrase IV at 2.8-A resolution

Proceedings of the National Academy of Sciences of the United States of America
T StamsD W Christianson

Abstract

It has recently been demonstrated that the C-terminal deletion mutant of recombinant human carbonic anhydrase IV (G267X CA IV) converts the normally glycosylphosphatidylinositol-anchored enzyme into a soluble secretory form which has the same catalytic properties as the membrane-associated enzyme purified from human tissues. We have determined the three-dimensional structure of the secretory form of human CA IV by x-ray crystallographic methods to a resolution of 2.8 A. Although the zinc binding site and the hydrophobic substrate binding pocket of CA IV are generally similar to those of other mammalian isozymes, unique structural differences are found elsewhere in the active site. Two disufide linkages, Cys-6-Cys-11G and Cys-23-Cys-203, stabilize the conformation of the N-terminal domain. The latter disulfide additionally stabilizes an active site loop containing a cis-peptide linkage between Pro-201 and Thr-202 (this loop contains catalytic residue Thr-199). On the opposite side of the active site, the Val-131-Asp-136 segment adopts an extended loop conformation instead of an alpha-helix conformation as found in other isozymes. Finally, the C terminus is surrounded by a substantial electropositive surface potential, which is l...Continue Reading

References

Dec 14, 1976·Biochemistry·W J Albery, J R Knowles
Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T OkuyamaW S Sly
Jan 1, 1992·Advances in Genetics·R E Tashian
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G S HagemanW S Sly
Jul 22, 1991·Biochimica Et Biophysica Acta·W F BrechueT H Maren
Mar 20, 1991·Journal of Molecular Biology·M W MacArthur, J M Thornton
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S SatoW S Sly
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D BrownW S Sly
Jul 1, 1990·The Journal of Membrane Biology·M P LisantiA R Saltiel
Jul 5, 1990·Journal of Molecular Biology·D E StewartJ E Wampler
Mar 1, 1989·Kidney International·P J Wistrand, K G Knuuttila
Feb 2, 1972·Nature: New Biology·A LiljasM Petef
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jan 1, 1984·Annals of the New York Academy of Sciences·K K KannanT A Jones
Jul 1, 1981·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·R M EffrosP Silverman
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·P A Boriack-SjodinD W Christianson
Jan 1, 1995·Annual Review of Biochemistry·W S Sly, P Y Hu
Jan 1, 1994·Protein Science : a Publication of the Protein Society·G M SmithC N Habecker
Jan 1, 1993·Annual Review of Biochemistry·P T Englund
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1993·Acta Crystallographica. Section D, Biological Crystallography·A T Brünger
Jan 23, 1987·Science·A T BrüngerM Karplus

❮ Previous
Next ❯

Citations

Dec 26, 2001·Journal of Inorganic Biochemistry·Changlin Liu, Huibi Xu
Nov 11, 1999·Biochimica Et Biophysica Acta·M QianD N Silverman
Oct 6, 2000·Journal of Molecular Graphics & Modelling·E X EspositoJ D Madura
Jul 17, 1998·Current Opinion in Chemical Biology·J E Coleman
Apr 5, 2007·Journal of the American Chemical Society·D K SrivastavaDavid W Christianson
Feb 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Rupak DattaWilliam S Sly
May 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Anna Di FioreGiuseppina De Simone
Nov 14, 2000·Journal of Bacteriology·K S SmithJ G Ferry
Aug 28, 2014·BioMed Research International·Rohan A DavisSally-Ann Poulsen
Oct 26, 2001·Biochemical and Biophysical Research Communications·T WingoD N Silverman
Aug 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Giuseppe BonapaceWilliam S Sly
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A WhittingtonD W Christianson
May 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lakshmanane PremkumarAda Zamir
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·S TamaiW S Sly
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Vincenzo AlterioGiuseppina De Simone
Jul 18, 2015·International Journal of Molecular Sciences·Anna Di FioreKatia D'Ambrosio
Oct 27, 2004·Journal of Enzyme Inhibition and Medicinal Chemistry·Silvia PastorekovaClaudiu T Supuran
Nov 10, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Mayank AggarwalRobert McKenna
Sep 9, 2000·FEMS Microbiology Reviews·K S Smith, J G Ferry
Jul 9, 2016·Expert Opinion on Therapeutic Patents·Carrie Lomelino, Robert McKenna
Oct 22, 2016·Journal of Enzyme Inhibition and Medicinal Chemistry·Daria M MontiSimona M Monti
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C E Smith
Feb 3, 2005·Bioorganic & Medicinal Chemistry Letters·Alessio InnocentiClaudiu T Supuran
Oct 5, 2017·European Biophysics Journal : EBJ·Aurelija MickevičiūtėDaumantas Matulis
Dec 25, 2002·Medicinal Research Reviews·Claudiu T SupuranAngela Casini
Nov 18, 2018·The Journal of Biological Chemistry·Linda S Forero-QuinteroHolger M Becker
Mar 18, 2010·Angewandte Chemie·Andreas Dunkel, Thomas Hofmann
Jul 14, 2010·Journal of Cellular Biochemistry·Aisha PandorSharon Prince
Sep 16, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·K M GilmourS F Perry
Jun 8, 2019·Expert Opinion on Therapeutic Patents·Sabina ZamanovaMarc A Ilies
Aug 23, 2019·Expert Opinion on Drug Discovery·Alessio Nocentini, Claudiu T Supuran

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.