Crystal structure of the superfamily 1 helicase from Tomato mosaic virus.

Journal of Virology
Masaki NishikioriEtsuko Katoh

Abstract

The genomes of the Tomato mosaic virus and many other plant and animal positive-strand RNA viruses of agronomic and medical importance encode superfamily 1 helicases. Although helicases play important roles in viral replication, the crystal structures of viral superfamily 1 helicases have not been determined. Here, we report the crystal structure of a fragment (S666 to Q1116) of the replication protein from Tomato mosaic virus. The structure reveals a novel N-terminal domain tightly associated with a helicase core. The helicase core contains two RecA-like α/β domains without any of the accessory domain insertions that are found in other superfamily 1 helicases. The N-terminal domain contains a flexible loop, a long α-helix, and an antiparallel six-stranded β-sheet. On the basis of the structure, we constructed deletion mutants of the S666-to-Q1116 fragment and performed split-ubiquitin-based interaction assays in Saccharomyces cerevisiae with TOM1 and ARL8, host proteins that are essential for tomato mosaic virus RNA replication. The results suggested that both TOM1 and ARL8 interact with the long α-helix in the N-terminal domain and that TOM1 also interacts with the helicase core. Prediction of secondary structures in other vi...Continue Reading

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Citations

Oct 25, 2012·PLoS Pathogens·Kazuhiro IshibashiMasayuki Ishikawa
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro IshibashiEtsuko Katoh
Oct 23, 2012·Current Opinion in Virology·Akira Mine, Tetsuro Okuno
Oct 16, 2012·Current Opinion in Virology·Kazuhiro IshibashiMasayuki Ishikawa
Apr 9, 2016·Cellular Logistics·Divya KhatterMahak Sharma
Dec 17, 2014·Virus Research·Kathleen C LehmannAlexander E Gorbalenya
Feb 17, 2015·Virus Research·Tetsuya ChujoMasayuki Ishikawa
Dec 18, 2013·Virus Research·Vaibhav MhaindarkarKavita S Lole
Sep 13, 2014·Current Opinion in Virology·Kazuhiro Ishibashi, Masayuki Ishikawa
Jun 15, 2016·Annual Review of Phytopathology·Kazuhiro Ishibashi, Masayuki Ishikawa
Jul 22, 2016·Journal of Virology·Changqing LiBruno Canard
Oct 2, 2020·International Journal of Molecular Sciences·Xiangyang LiDeyu Hu
Apr 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yee-Song LawDahai Luo
Jul 22, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nuruddin UnchwaniwalaPaul Ahlquist

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