Crystal structure of the transcriptional repressor PagR of Bacillus anthracis.

Microbiology
Haiyan ZhaoKottayil I Varughese

Abstract

PagR is a transcriptional repressor in Bacillus anthracis that controls the chromosomal S-layer genes eag and sap, and downregulates the protective antigen pagA gene by direct binding to their promoter regions. The PagR protein sequence is similar to those of members of the ArsR repressor family involved in the repression of arsenate-resistance genes in numerous bacteria. The crystal structure of PagR was solved using multi-wavelength anomalous diffraction (MAD) techniques and was refined with 1.8 A resolution diffraction data. The PagR molecules form dimers, as observed in all SmtB/ArsR repressor family proteins. In the crystal lattice four PagR dimers pack together to form an inactive octamer. Model-building studies suggest that the dimer binds to a DNA duplex with a bend of around 4 degrees.

References

Sep 5, 1992·Journal of Molecular Biology·L J Beamer, C O Pabo
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 11, 1990·Nucleic Acids Research·I B Dodd, J B Egan
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jul 27, 1999·Journal of Bacteriology·A R Hoffmaster, T M Koehler
Jun 1, 2000·Applied and Environmental Microbiology·E HelgasonA B Kolstø
Apr 10, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Laura S BusenlehnerDavid P Giedroc
Apr 16, 2002·Molecular Microbiology·Tâm MignotAgnès Fouet
Sep 28, 2002·Nature Structural Biology·Ann E MarisRichard E Dickerson
Oct 22, 2003·Journal of Molecular Biology·Christoph EickenDavid P Giedroc
Dec 31, 2003·Methods in Enzymology·Thomas C Terwilliger
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Andrew G W Leslie
Mar 28, 2008·Molecular Microbiology·Justin W Kern, Olaf Schneewind
Aug 1, 2008·Microbiology·Maria Hadjifrangiskou, Theresa M Koehler
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Apr 5, 2013·PloS One·Stephanie AbromaitisJane E Koehler
Sep 25, 2010·Research in Microbiology·Agnès Fouet
May 18, 2017·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Rudra P SahaManoj Kumar Singh
May 11, 2017·Essays in Biochemistry·Daiana A CapdevilaDavid P Giedroc

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