Crystal structure of tubulin folding cofactor A from Arabidopsis thaliana and its beta-tubulin binding characterization

FEBS Letters
Lu LuYi Li

Abstract

Microtubules are composed of polymerized alpha/beta-tubulin heterodimers. Biogenesis of assembly-competent tubulin dimers is a complex multistep process that requires sequential actions of distinct molecular chaperones and cofactors. Tubulin folding cofactor A (TFCA), which captures beta-tubulin during the folding pathway, has been identified in many organisms. Here, we report the crystal structure of Arabidopsis thaliana TFC A (KIESEL, KIS), which forms a monomeric three-helix bundle. The functional binding analysis demonstrated that KIS interacts with beta-tubulin in plant. Furthermore, mutagenesis studies indicated that the alpha-helical regions of KIS participate in beta-tubulin binding. Unlike the budding yeast TFC A, the two loop regions of KIS are not required for this interaction suggesting a distinct binding mechanism of TFC A to beta-tubulin in plants.

References

Oct 1, 1990·The Plant Cell·M A RestrepoJ C Carrington
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H RommelaereC Ampe
Jun 1, 1994·The Journal of Cell Biology·Y GaoN J Cowan
Jul 26, 1996·Cell·G TianN J Cowan
Feb 16, 1999·Cell·E NogalesK H Downing
May 13, 1999·Bioinformatics·P GouetF Métoz
Jul 13, 1999·Cell Motility and the Cytoskeleton·M L FanarragaJ C Zabala
Dec 14, 1999·Nature Structural Biology·S Steinbacher
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·T H TaiB J Staskawicz
Oct 3, 2001·Journal of Structural Biology·M Lopez-FanarragaJ C Zabala
Dec 12, 2001·Molecular and Cellular Biology·Katharine C AbruzziFrank Solomon
Jun 11, 2002·Journal of Molecular Biology·Alicia GuaschMiquel Coll
Sep 13, 2002·Current Biology : CB·Victor KirikMartin Hülskamp
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Aug 24, 2004·Journal of Molecular Biology·Liru YouTim C Huffaker
Oct 8, 2004·The Plant Journal : for Cell and Molecular Biology·Michael WalterJörg Kudla
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Aug 25, 2006·Plant & Cell Physiology·Pankaj DhonuksheTheodorus W J Gadella
Aug 22, 2007·Trends in Cell Biology·S A LewisN J Cowan
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ying GuChris Somerville
Dec 29, 2009·Frontiers in Bioscience (Elite Edition)·Yunlong DuYi Li
Aug 10, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Lu LuYi Li

❮ Previous
Next ❯

Citations

Jun 10, 2011·Cellular and Molecular Life Sciences : CMLS·Ewa Szolajska, Jadwiga Chroboczek
Oct 14, 2000·Annual Review of Cell and Developmental Biology·K H Downing
Feb 1, 2011·Plant, Cell & Environment·Songhu WangJan A Smalle
Sep 4, 2015·Acta Crystallographica. Section D, Biological Crystallography·Min Kyu KimSa Ouk Kang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.