Crystal structure of unphosphorylated STAT3 core fragment

Biochemical and Biophysical Research Communications
Zhiyong RenXiaomin Chen

Abstract

Signal transducers and activators of transcription (STATs) are latent cytoplasmic transcriptional factors that play an important role in cytokine and growth factor signaling. Here we report a 3.05 A-resolution crystal structure of an unphosphorylated STAT3 core fragment. The overall monomeric structure is very similar to that of the phosphorylated STAT3 core fragment. However, the dimer interface observed in the unphosphorylated STAT1 core fragment structure is absent in the STAT3 structure. Solution studies further demonstrate that the core fragment of STAT3 is primarily monomeric. Mutations corresponding to those in STAT1, which lead to disruption of the core fragment interface and prolonged tyrosine phosphorylation, show little or no effect on the tyrosine phosphorylation kinetics of STAT3. These results highlight the structural and biochemical differences between STAT3 and STAT1, and suggest different regulation mechanisms of these two proteins.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 1, 1997·Molecular and Cellular Biology·T S SchaeferD Nathans
Jun 13, 2001·Methods in Enzymology·J Bromberg, X Chen
May 2, 2002·Cell·John J O'SheaRobert D Schreiber
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·David E Levy, J E Darnell
Jul 2, 2003·The Journal of Biological Chemistry·Jutta BraunsteinChristian Schindler
Jan 6, 2004·Nature Immunology·Naruhisa OtaKenneth M Murphy
Mar 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Minghao ZhongJames E Darnell
Mar 23, 2005·Molecular Cell·Xiang MaoXiaomin Chen
Apr 5, 2005·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read
Sep 30, 2005·The Journal of Biological Chemistry·Dante NeculaiStefan Becker
May 24, 2006·Molecular and Cellular Biochemistry·Ling ZhangStephanie S Watowich
Jan 12, 2007·Molecular BioSystems·Cheh Peng Lim, Xinmin Cao
Nov 17, 2007·Nature Protocols·Axel T Brunger
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jun 4, 2013·Seminars in Cancer Biology·Federico PietrocolaGuido Kroemer
Apr 17, 2012·Journal of Chemical Information and Modeling·Jarmila HusbyStephen Neidle
Jun 28, 2011·Journal of Medicinal Chemistry·Tamás KálaiKálmán Hideg
Jun 2, 2012·Journal of Medicinal Chemistry·Bikash DebnathNouri Neamati
Mar 2, 2011·Journal of Molecular Recognition : JMR·In-Hee Park, Chenglong Li
Apr 1, 2015·The FEBS Journal·Matthew S Wake, Christine J Watson
Oct 25, 2011·European Journal of Cell Biology·Anne MohrGerhard Müller-Newen
Jul 23, 2015·Journal of Immunology Research·Suresh V Kuchipudi
Dec 18, 2013·Cell Cycle·Barry Jutten, Kasper M A Rouschop
Oct 23, 2012·Molecular Cell·Shensi ShenGuido Kroemer
Mar 11, 2015·Journal of Computer-aided Molecular Design·Eleonora Gianti, Randy J Zauhar
Jun 28, 2016·The Journal of Allergy and Clinical Immunology·Prabha ChandrasekaranSteven M Holland
Sep 4, 2016·Protein Expression and Purification·Elvin D de AraujoPatrick T Gunning
Sep 24, 2016·Molecular Neurobiology·Zangbéwendé Guy OuédraogoEmmanuel Chautard
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jing LiSongying Ouyang
May 31, 2018·International Journal of Molecular Sciences·Jacopo SgrignaniAndrea Cavalli
Jul 15, 2015·Molecular and Cellular Biology·Tiancen HuDavid A Frank
Sep 30, 2018·Protein Science : a Publication of the Protein Society·Rhiannon MorrisJeffrey J Babon
Nov 6, 2018·Journal of Cellular and Molecular Medicine·Zhaojuan YangJi Wu
Feb 19, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xu ChenCalvin Yu-Chian Chen
Aug 4, 2016·The Biochemical Journal·Elena ButturiniSofia Mariotto
Apr 15, 2020·Organic & Biomolecular Chemistry·Matthew B MinusZachary T Ball
May 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xin QiJing Li
May 15, 2018·Nucleic Acids Research·Min HuangJian Zhang
Nov 30, 2019·Oxidative Medicine and Cellular Longevity·Elena ButturiniSofia Mariotto
May 12, 2018·International Journal of Oncology·Yin ShiXia Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.