Crystal structure of VnfH, the iron protein component of vanadium nitrogenase

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Michael RohdeOliver Einsle

Abstract

Nitrogenases catalyze the biological fixation of inert N2 into bioavailable ammonium. They are bipartite systems consisting of the catalytic dinitrogenase and a complementary reductase, the Fe protein that is also the site where ATP is hydrolyzed to drive the reaction forward. Three different subclasses of dinitrogenases are known, employing either molybdenum, vanadium or only iron at their active site cofactor. Although in all these classes the mode and mechanism of interaction with Fe protein is conserved, each one encodes its own orthologue of the reductase in the corresponding gene cluster. Here we present the 2.2 Å resolution structure of VnfH from Azotobacter vinelandii, the Fe protein of the alternative, vanadium-dependent nitrogenase system, in its ADP-bound state. VnfH adopts the same conformation that was observed for NifH, the Fe protein of molybdenum nitrogenase, in complex with ADP, representing a state of the functional cycle that is ready for reduction and subsequent nucleotide exchange. The overall similarity of NifH and VnfH confirms the experimentally determined cross-reactivity of both ATP-hydrolyzing reductases.

References

Nov 10, 1995·The Journal of Biological Chemistry·J W PetersD R Dean
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·D W Cruickshank
May 21, 1999·Acta Crystallographica. Section D, Biological Crystallography·J G GrossmanD C Rees
Nov 7, 1996·Chemical Reviews·James B. Howard, Douglas C. Rees
Nov 7, 1996·Chemical Reviews·Barbara K. Burgess, David J. Lowe
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E BlancG Bricogne
May 20, 2005·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Douglas C ReesJames B Howard
Jun 1, 1930·Journal of Bacteriology·D Burk, H Lineweaver
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Mar 12, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Yilin HuMarkus W Ribbe
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 7, 2010·Science·Chi Chung LeeMarkus W Ribbe
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 20, 2011·Coordination Chemistry Reviews·Yilin Hu, Markus W Ribbe
May 14, 2011·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Scott E MottarellaPaul A Craig
Sep 24, 2011·Biochemistry·Karamatullah DanyalLance C Seefeldt
Nov 19, 2011·Science·Thomas SpatzalOliver Einsle
May 26, 2012·Science·P Andrew Karplus, Kay Diederichs
Jan 29, 2014·Chemical Reviews·Brian M HoffmanLance C Seefeldt
Feb 22, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Oliver Einsle
Sep 12, 2015·Journal of the American Chemical Society·Cedric P OwensF Akif Tezcan
Dec 15, 2015·Journal of the American Chemical Society·Julia SchlesierOliver Einsle
Oct 5, 2016·Proceedings of the National Academy of Sciences of the United States of America·Karamatullah DanyalEdwin Antony
Dec 9, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Daniel SippelOliver Einsle
Jul 12, 2017·Nature Chemical Biology·Daniel Sippel, Oliver Einsle
Aug 25, 2017·Protein Science : a Publication of the Protein Society·Elizabeth JurrusNathan A Baker
Jan 18, 2018·Nature Microbiology·Yanning ZhengCaroline S Harwood
Mar 31, 2018·Science·Daniel SippelOliver Einsle

❮ Previous
Next ❯

Citations

Oct 6, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lawrence Que
Sep 12, 2020·Angewandte Chemie·Michael RohdeOliver Einsle
Mar 5, 2020·Chemical Reviews·Andrew J JasniewskiYilin Hu
Mar 17, 2020·Chemical Reviews·Lance C SeefeldtBrian M Hoffman
Apr 3, 2020·Chemical Reviews·Casey Van StappenSerena DeBeer
Oct 22, 2020·Journal of Inorganic Biochemistry·Natasha PenceJohn W Peters
Oct 18, 2020·Journal of Inorganic Biochemistry·Derek F HarrisLance C Seefeldt
Jun 17, 2020·Chemical Reviews·Oliver Einsle, Douglas C Rees

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray

Software Mentioned

COOT
Phaser
TNT
AIMLESS
APBS
PyMOL
XDS
CCP4
PROCHECK
Buster

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.