Crystal Structure of Xanthomonas AvrRxo1-ORF1, a Type III Effector with a Polynucleotide Kinase Domain, and Its Interactor AvrRxo1-ORF2

Structure
Qian HanBingyu Zhao

Abstract

Xanthomonas oryzae pv. oryzicola (Xoc) causes bacterial leaf streak (BLS) disease on rice plants. Xoc delivers a type III effector AvrRxo1-ORF1 into rice plant cells that can be recognized by disease resistance (R) protein Rxo1, and triggers resistance to BLS disease. However, the mechanism and virulence role of AvrRxo1 is not known. In the genome of Xoc, AvrRxo1-ORF1 is adjacent to another gene AvrRxo1-ORF2, which was predicted to encode a molecular chaperone of AvrRxo1-ORF1. We report the co-purification and crystallization of the AvrRxo1-ORF1:AvrRxo1-ORF2 tetramer complex at 1.64 Å resolution. AvrRxo1-ORF1 has a T4 polynucleotide kinase domain, and expression of AvrRxo1-ORF1 suppresses bacterial growth in a manner dependent on the kinase motif. Although AvrRxo1-ORF2 binds AvrRxo1-ORF1, it is structurally different from typical effector-binding chaperones, in that it has a distinct fold containing a novel kinase-binding domain. AvrRxo1-ORF2 functions to suppress the bacteriostatic activity of AvrRxo1-ORF1 in bacterial cells.

References

Jun 30, 2000·Current Opinion in Plant Biology·J EllisT Pryor
Apr 13, 2001·Bioinformatics·C Gille, C Frömmel
May 4, 2001·The Journal of Biological Chemistry·L K Wang, S Shuman
Jul 12, 2002·The EMBO Journal·Li Kai WangStewart Shuman
Dec 6, 2002·Genome Research·Jianfa BaiScot H Hulbert
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anton MeinhartWolfram Saenger
Mar 5, 2003·Current Opinion in Microbiology·Claude ParsotAnne-Laure Page
Apr 3, 2003·The Plant Cell·Blake C MeyersRichard W Michelmore
Feb 3, 2004·Nucleic Acids Research·Jennifer H EastbergBarry L Stoddard
Mar 16, 2004·Structure·Christian C LeeFumiaki Katagiri
Apr 29, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B Y ZhaoS H Hulbert
Jul 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jeff H ChangJeffery L Dangl
Mar 8, 2005·Molecular Cell·Nina K BernsteinJ N Mark Glover
Mar 9, 2005·Current Opinion in Plant Biology·Blake C MeyersRaja Sekhar Nandety
Sep 24, 2005·Nature Immunology·Frederick M Ausubel
Oct 7, 2005·Nature·Yukihiro Akeda, Jorge E Galán
Oct 19, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bingyu ZhaoScot Hulbert
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Feb 25, 2006·Cell·Stephen T ChisholmBrian J Staskawicz
Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Robert B AbramovitchGregory B Martin
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Jul 29, 2008·Current Opinion in Plant Biology·Anna BlockJames R Alfano
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jan 9, 2010·Acta Crystallographica. Section D, Biological Crystallography·Vincent B ChenDavid C Richardson
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
May 1, 2010·Nature Protocols·Sjoerd J de VriesAlexandre M J J Bonvin
Sep 1, 2006·Molecular Plant Pathology·David O Niño-LiuAdam J Bogdanove
Nov 11, 2010·Molecular Plant-microbe Interactions : MPMI·Dor SalomonGuido Sessa

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Citations

Jun 20, 2017·PLoS Pathogens·Teja ShidoreLindsay R Triplett
Aug 22, 2017·Frontiers in Microbiology·Ramón Díaz-OrejasChew Chieng Yeo
Sep 14, 2016·The Journal of Biological Chemistry·Felix SchuebelAnton Meinhart
May 20, 2017·Annual Review of Phytopathology·T Shidore, L R Triplett
Jan 13, 2019·Toxins·María Moreno-Del ÁlamoJuan C Alonso
Oct 4, 2018·International Journal of Molecular Sciences·Zhiyuan JiKaijun Zhao
Jun 3, 2021·International Journal of Molecular Sciences·Anastasia D GaziVasiliki E Fadouloglou
Jul 6, 2021·FEMS Microbiology Reviews·Morgane Roussin, Suzana P Salcedo

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